| Molecular characterization of Mycobacterium tuberculosis isolates from different regions of Bulgaria. | |
| | |
MedLine Citation:
|
PMID: 18199794 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Mycobacterium tuberculosis isolates from different regions of Bulgaria were studied by a variety of molecular typing tools. Based on spacer oligonucleotide typing (spoligotyping), the 113 strains were subdivided into 35 spoligotypes: 5 unique profiles and 15 profiles shared by two to 29 strains; the Hunter-Gaston diversity index (HGI) was 0.9. Comparison with the international database SITVIT2 at the Institut Pasteur de Guadeloupe showed the presence of two globally distributed shared types, ST53 (25.7%) and ST47 (6.2%). Nineteen (16.8%) and six (5.3%) strains belonged to the ST125 (LAM/S subfamily) and ST41 (LAM7_TUR subfamily) types described in SITVIT2 as ubiquitous/rare and ubiquitous/common types, respectively. Seven spoligoprofiles (12 strains) were not found in the database; two of them constituted new shared types. The Beijing genotype strains were not found in the studied collection in spite of close contacts with Russia in the recent and historical past. Additional subtyping by IS6110-restriction fragment length polymorphism (RFLP) and 12-locus mycobacterial interspersed repetitive unit (MIRU)-variable number of tandem repeat analyses were performed within selected spoligotypes. In particular, MIRU typing showed better discrimination within ST125 than IS6110-RFLP typing (HGI = 0.83 versus 0.39). A high gradient for ST125 in Bulgaria compared to its negligible presence in the global database and neighboring countries leads us to suggest a Bulgarian phylogeographic specificity of this spoligotype. To conclude, this first study of the Bulgarian M. tuberculosis population demonstrated its heterogeneity and predominance of several worldwide-distributed and Balkan-specific spoligotypes. |
| | |
Authors:
|
Violeta Valcheva; Igor Mokrousov; Nalin Rastogi; Olga Narvskaya; Nadya Markova |
Related Documents
:
|
17626174 - Outbreak of mycobacterium massiliense infection associated with intramuscular injections. 3424264 - Combined versus single antituberculosis drugs on the in vitro sensitivity patterns of n... 1685494 - Occurrence and stability of insertion sequences in mycobacterium tuberculosis complex s... 15273144 - Rv2686c-rv2687c-rv2688c, an abc fluoroquinolone efflux pump in mycobacterium tuberculosis. 1617074 - Enterococcal endocarditis. 20047104 - Vaca genotypes in helicobacter pylori strains isolated from patients with and without d... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-01-16 |
Journal Detail:
|
Title: Journal of clinical microbiology Volume: 46 ISSN: 1098-660X ISO Abbreviation: J. Clin. Microbiol. Publication Date: 2008 Mar |
Date Detail:
|
Created Date: 2008-03-10 Completed Date: 2008-04-11 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 7505564 Medline TA: J Clin Microbiol Country: United States |
Other Details:
|
Languages: eng Pagination: 1014-8 Citation Subset: IM |
Affiliation:
|
Department of Pathogenic Bacteria, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Bacterial Typing Techniques* Bulgaria / epidemiology DNA Fingerprinting / methods DNA Transposable Elements / genetics Humans Interspersed Repetitive Sequences / genetics Minisatellite Repeats / genetics Mycobacterium tuberculosis / classification*, genetics*, isolation & purification Oligonucleotides / analysis Polymorphism, Restriction Fragment Length / genetics Tuberculosis / epidemiology*, microbiology* |
| Chemical | |
Reg. No./Substance:
|
0/DNA Transposable Elements; 0/Oligonucleotides |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Bacteremia and infective endocarditis caused by a non-daptomycin-susceptible, vancomycin-intermediat...
Next Document: 2007 Homer W. Smith award: control of terminal differentiation in epithelia.