Document Detail


Molecular characterization of Echinococcus granulosus in south-eastern Romania: evidence of G1-G3 and G6-G10 complexes in humans.
MedLine Citation:
PMID:  22967309     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Clin Microbiol Infect ABSTRACT: Echinococcus granulosus is the aetiological agent of cystic echinococcosis (CE), which is a public health problem in many eastern European countries, particularly in Romania, where the infection causes a high number of human and animal cases. To shed light on the transmission patterns of the parasite, we performed a genotyping analysis on 60 cyst samples obtained from patients who live in south-eastern Romania and who underwent surgery for liver or lung CE. DNA was extracted from the endocysts or the cyst fluids, and fragments of cytochrome c oxidase subunit 1 and NADH dehydrogenase subunit 1 mitochondrial genes (cox1 and nd1, respectively) were amplified by PCR and sequenced. We found that most of the samples analysed (59/60) belonged to the G1-G3 complex (E. granulosus sensu stricto), which contains the most widespread and infective strains of the parasite. We also identified the first human patient infected by a non-G1-G3 genotype of E. granulosus in this country. As the DNA sequence of this cyst sample showed maximum homology with the G6-G10 complex (Echinococcus canadensis), this is, in all likelihood, a G7 genotype, which is often found in pigs and dogs in most countries of eastern and south-eastern Europe.
Authors:
L Piccoli; C Bazzocchi; E Brunetti; P Mihailescu; C Bandi; B Mastalier; I Cordos; M Beuran; L G Popa; V Meroni; F Genco; C Cretu
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-17
Journal Detail:
Title:  Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases     Volume:  -     ISSN:  1469-0691     ISO Abbreviation:  Clin. Microbiol. Infect.     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-9-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9516420     Medline TA:  Clin Microbiol Infect     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 The Authors. Clinical Microbiology and Infection © 2012 European Society of Clinical Microbiology and Infectious Diseases.
Affiliation:
 Department of Infectious Diseases, IRCCS San Matteo Hospital Foundation, WHO Collaborating Centre for Clinical Management of Cystic Echinococcosis, University of Pavia, Pavia  Department of Veterinary Science and Public Health, School of Veterinary Medicine, University of Milan, Milan, Italy  Eco-Para-Diagnostic Society, Laboratory of Parasitology  Department of Surgery, Colentina Clinical Hospital  Department of Thoracic Surgery, Marius Nasta Institute of Pneumology  Department of Surgery, Floreasca Emergency Clinical Hospital  Department of Parasitology, Colentina Clinical Hospital, 'Carol Davila' University of Medicine, Bucharest, Romania  Laboratory of Parasitology, Department of Infectious Diseases, IRCCS San Matteo Hospital Foundation, University of Pavia, Pavia, Italy.
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