| Molecular characterization of a 7p15-21 homozygous deletion in a Wilms tumor. | |
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MedLine Citation:
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PMID: 12461744 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Recent molecular studies have shown a relatively high rate of loss of heterozygosity (LOH) at band 7p15-21 in Wilms tumor. We previously reported that the minimal common region of LOH was located between markers D7S517 and D7S503 in bands 7p15-21. We also reported the identification of one Wilms tumor (GOS44) bearing a homozygous, interstitial deletion at a locus within this region. Homogeneous primary cell cultures have been derived from this tumor and have been used for all the subsequent analyses. Using PCR and a panel of STS markers mapping between D7S517 and D7S503, the physical boundaries of the homozygous deletion were determined to be between D7S638 and D7S644. The deleted region spans approximately 3 Mbp of genomic sequence and includes seven known genes (KIAA0744, KIAA0713, AHR, AGR2, NET6, HSPC028, and DGKB.) as well as five predicted genes with similarities to genes of known function (LOC-91802, -116364, -96009, -92511, and -92512). The proximal breakpoint was found to lie between exon 6 and exon 7 of KIAA0744, and the distal breakpoint lay between exon 17 and exon 18 of DGKB. It is unlikely that a functional fusion gene product was generated as a consequence of the fusion between these two genes, because they are oriented in opposite directions on the chromosome. This is the only reported homozygous deletion recorded so far in Wilms tumor, and it provides the means to identify the tumor-suppressor gene located in this deletion. |
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Authors:
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Khalid Sossey-Alaoui; Luís Vieira; Dezso David; Maria G Boavida; John K Cowell |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Genes, chromosomes & cancer Volume: 36 ISSN: 1045-2257 ISO Abbreviation: Genes Chromosomes Cancer Publication Date: 2003 Jan |
Date Detail:
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Created Date: 2002-12-03 Completed Date: 2003-01-17 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9007329 Medline TA: Genes Chromosomes Cancer Country: United States |
Other Details:
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Languages: eng Pagination: 1-6 Citation Subset: IM |
Copyright Information:
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Copyright 2002 Wiley-Liss, Inc. |
Affiliation:
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Department of Cancer Genetics, Roswell Park Cancer Institute, Buffalo, New York 14263, USA. khalid.sossey-alaoui@roswellpark.org |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromosome Deletion* Chromosomes, Human, Pair 7 / genetics* DNA Mutational Analysis / methods DNA, Neoplasm / genetics Diacylglycerol Kinase / genetics Genes, Wilms Tumor Genetic Markers / genetics Histone Deacetylases* Humans Loss of Heterozygosity / genetics* Physical Chromosome Mapping / methods Repressor Proteins / genetics Tumor Cells, Cultured Wilms Tumor / enzymology, genetics*, pathology |
| Grant Support | |
ID/Acronym/Agency:
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P30CA16056/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA, Neoplasm; 0/Genetic Markers; 0/Repressor Proteins; EC 2.7.1.107/Diacylglycerol Kinase; EC 3.5.1.98/HDAC9 protein, human; EC 3.5.1.98/Histone Deacetylases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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