Document Detail


Molecular characterization, 3D modelling and expression analysis of sea bass (Dicentrarchus labrax L.) interleukin-10.
MedLine Citation:
PMID:  17049605     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Interleukin-10 (IL-10) is a pleiotropic cytokine generally known for its relevance in the resolution of inflammation, but that also has immunostimulatory properties. Here is described the isolation and characterization of the sea bass IL-10 (sbIL-10) cDNA and gene. The sbIL-10 gene encodes a 187 amino acid protein and comprises a five exon-four intron structure as other known IL-10 genes. Important structural residues are maintained in the sbIL-10 protein, including the four cysteines responsible for the two intra-chain disulfide bridges reported for human IL-10. The 3D structure of sbIL-10 was predicted. This first homology model of a fish IL-10 reveals a high degree of compatibility between the dimeric quaternary architectures of sbIL-10 and its mammalian counterparts. The phylogenetic analysis clusters sbIL-10 with other IL-10s, apart from IL-10-related molecules. The involvement of IL-10 in sea bass immune responses was demonstrated by investigating the expression profiles of IL-1beta and IL-10 in the head-kidney and spleen following intraperitoneal injection of UV-killed Photobacterium damselae ssp. piscicida. Furthermore, involvement of IL-10 in the resolution of inflammation is for the first time suggested in fish, due to the delayed maximal mRNA levels of sbIL-10 compared to those of the pro-inflammatory IL-1beta.
Authors:
Rute D Pinto; Diana S Nascimento; Marta I R Reis; Ana do Vale; Nuno M S Dos Santos
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-10-17
Journal Detail:
Title:  Molecular immunology     Volume:  44     ISSN:  0161-5890     ISO Abbreviation:  Mol. Immunol.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2006-12-04     Completed Date:  2007-03-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7905289     Medline TA:  Mol Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  2056-65     Citation Subset:  IM    
Affiliation:
IBMC-Instituto de Biologia Molecular e Celular, Rua do Campo Alegre 823, 4150-180 Porto, Portugal.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Bass / genetics*,  immunology
Cloning, Molecular
Gene Expression Regulation / physiology*
Interleukin-10 / chemistry,  genetics*,  immunology
Models, Molecular*
Molecular Sequence Data
Organ Specificity / physiology
Protein Structure, Tertiary
Chemical
Reg. No./Substance:
130068-27-8/Interleukin-10

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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