| Molecular characteristics of extended-spectrum beta-lactamase-producing Escherichia coli from the Chicago area: high prevalence of ST131 producing CTX-M-15 in community hospitals. | |
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MedLine Citation:
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PMID: 20359869 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study was designed to characterise 30 non-duplicate extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli clinical isolates from the community in the Chicago metropolitan area collected during 2008. The majority of isolates (n=28) were recovered from urine and 2 isolates were from blood. Molecular characterisation was done using the following techniques: isoelectric focusing; polymerase chain reaction (PCR) and sequencing of bla(ESBL); PCR for plasmid-mediated quinolone resistance determinants; identification of ST131; phylogenetic grouping; and replicon typing. Genetic relatedness was determined by pulsed-field gel electrophoresis (PFGE) with XbaI and repetitive sequence-based PCR (rep-PCR) typing. Twenty-six (87%) of the ESBL-producing E. coli were positive for bla(CTX-M) genes (22 CTX-M-15 and 4 CTX-M-14), whilst the remaining 4 isolates produced SHV-2. Twenty-eight isolates (93%) were non-susceptible to ciprofloxacin and 16 (53%) were positive for aac(6')-Ib-cr. Overall, 16 (53%) of the ESBL-producers belonged to clonal complex ST131 that produced CTX-M-15 or CTX-M-14. Molecular characteristics of ST131 showed that it belonged to three distinct but related PFGE clones, was derived from phylogenetic group B2 and contained IncFII type plasmids. These results illustrate that E. coli clonal complex ST131 producing CTX-M-15, CTX-M-14, OXA-1, TEM-1 and aac(6')-Ib-cr has emerged as an important cause of community-onset urinary tract infections caused by ESBL-producing E. coli isolates in the Chicago area. |
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Authors:
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Gisele Peirano; Mike Costello; Johann D D Pitout |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-31 |
Journal Detail:
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Title: International journal of antimicrobial agents Volume: 36 ISSN: 1872-7913 ISO Abbreviation: Int. J. Antimicrob. Agents Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-05-24 Completed Date: 2010-08-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9111860 Medline TA: Int J Antimicrob Agents Country: Netherlands |
Other Details:
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Languages: eng Pagination: 19-23 Citation Subset: IM |
Copyright Information:
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(c) 2010 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved. |
Affiliation:
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Division of Microbiology, Calgary Laboratory Services, Calgary, AB, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aged Bacteremia / epidemiology, microbiology Bacterial Typing Techniques Chicago / epidemiology Cluster Analysis Community-Acquired Infections / epidemiology*, microbiology* DNA Fingerprinting Electrophoresis, Gel, Pulsed-Field Escherichia coli / classification*, enzymology*, isolation & purification Escherichia coli Infections / epidemiology*, microbiology* Female Hospitals, Community Humans Isoelectric Focusing Male Molecular Epidemiology Plasmids / analysis Polymerase Chain Reaction Prevalence Sequence Analysis, DNA Urinary Tract Infections / epidemiology, microbiology beta-Lactamases / biosynthesis*, chemistry, genetics |
| Chemical | |
Reg. No./Substance:
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EC 3.5.2.6/beta-Lactamases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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