| Molecular characterisation and inductive expression of a fish protein arginine methyltransferase 1 gene in response to virus infection. | |
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MedLine Citation:
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PMID: 17055744 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Protein arginine methyltransferase 1 (PRMT1) is currently thought as an effector to regulate interferon (IFN) signalling. Here Paralichthys olivaceus PRMT1 (PoPRMT1) gene was identified as a virally induced gene from UV-inactivated Scophthalmus maximus Rhabdovirus (SMRV)-infected flounder embryonic cells (FEC). PoPMRT1 encodes a 341-amino-acid protein that shares the conserved domains including post-I, motif I, II and III. Homology comparisons show that the putative PoPMRT1 protein is the closest to zebrafish PMRT1 and belongs to type I PRMT family (including PRMT1, PRMT2, PRMT3, PRMT4, PRMT6, PRMT8). Expression analyses revealed an extensive distribution of PoPMRT1 in all tested tissues of flounder. In vitro induction of PoPRMT1 was determined in UV-inactivated SMRV-infected FEC cells, and under the same conditions, flounder Mx was also transcriptionally up-regulated, indicating that an IFN response might be triggered. Additionally, live SMRV infection of flounders induced an increased expression of PoPRMT1 mRNA and protein significantly in spleen, and to a lesser extent in head kidney and intestine. Immunofluorescence analysis revealed a major cyptoplasmic distribution of PoPRMT1 in normal FEC but an obvious increase occurred in nucleus in response to UV-inactivated SMRV. This is the first report on in vitro and in vivo expression of fish PRMT1 by virus infection, suggesting that PoPRMT1 might be implicated in flounder antiviral immune response. |
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Authors:
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Cai-Wen Dong; Yi-Bing Zhang; Ai-Jun Lu; Rong Zhu; Fu-Tie Zhang; Qi-Ya Zhang; Jian-Fang Gui |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-07-10 |
Journal Detail:
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Title: Fish & shellfish immunology Volume: 22 ISSN: 1050-4648 ISO Abbreviation: Fish Shellfish Immunol. Publication Date: 2007 Apr |
Date Detail:
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Created Date: 2006-12-25 Completed Date: 2007-05-10 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9505220 Medline TA: Fish Shellfish Immunol Country: England |
Other Details:
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Languages: eng Pagination: 380-93 Citation Subset: IM |
Affiliation:
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State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Wuhan 430072, China. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/DQ493595 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence Cell Line DNA Primers / chemistry Embryo, Nonmammalian / cytology Fish Diseases / enzymology, immunology*, virology Flounder / genetics, immunology*, virology Gene Expression Regulation, Enzymologic / immunology* Molecular Sequence Data Phylogeny Protein-Arginine N-Methyltransferases / biosynthesis, chemistry, genetics*, immunology RNA, Messenger Reverse Transcriptase Polymerase Chain Reaction / veterinary Rhabdoviridae / immunology Rhabdoviridae Infections / enzymology, immunology, veterinary* Sequence Alignment / veterinary Sequence Homology, Amino Acid Up-Regulation |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/RNA, Messenger; EC 2.1.1.-/Protein-Arginine N-Methyltransferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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