| Molecular characterisation of drug resistance in Mycobacterium tuberculosis isolates from North India. | |
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MedLine Citation:
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PMID: 23317963 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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SETTING: A tertiary care hospital in North India. OBJECTIVES: To analyse the frequency of mutations associated with resistance to rifampicin (RMP), isoniazid (INH) and streptomycin (SM) in Mycobacterium tuberculosis. DESIGN: M. tuberculosis isolates from 171 pulmonary tuberculosis (TB) patients (newly diagnosed 102, 59.6%; retreated 69, 40.3%) were analysed. Drug susceptibility testing was performed using the proportion method and resistant isolates were characterised using the polymerase chain reaction, followed by restriction fragment length polymorphism and/or DNA sequencing, to screen for mutations in rpoB, katG, mabA-inhA and rpsL. RESULTS: Of the 171 isolates, 16.9% (29/171) were multidrug-resistant. Of the 102 newly diagnosed and 69 retreated cases, respectively 5.9% (6/102) and 33.3% (23/69) were multidrug-resistant. rpoB mutations were found in 100% (31/31) of the RMP-resistant isolates, the most common being S531L in 74.2% (23/31); katG315 mutations were found in 79.6% (35/44) of the INH-resistant isolates; however, no mabA-inhA (-15) mutation was found; rpsL mutations were found in 48.9% (24/49) of the SM-resistant isolates, and codon 43 mutation were found in 42.5% (21/49). CONCLUSIONS: This study characterises drug resistance-associated mutations in M. tuberculosis, information that could be used for the rapid screening of drug-resistant TB. |
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Authors:
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R Yadav; S Sethi; S K Dhatwalia; D Gupta; A Mewara; M Sharma |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease Volume: 17 ISSN: 1815-7920 ISO Abbreviation: Int. J. Tuberc. Lung Dis. Publication Date: 2013 Feb |
Date Detail:
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Created Date: 2013-01-15 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9706389 Medline TA: Int J Tuberc Lung Dis Country: France |
Other Details:
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Languages: eng Pagination: 251-7 Citation Subset: IM |
Affiliation:
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Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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