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Molecular characterisation of drug resistance in Mycobacterium tuberculosis isolates from North India.
MedLine Citation:
PMID:  23317963     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
SETTING: A tertiary care hospital in North India.
OBJECTIVES: To analyse the frequency of mutations associated with resistance to rifampicin (RMP), isoniazid (INH) and streptomycin (SM) in Mycobacterium tuberculosis.
DESIGN: M. tuberculosis isolates from 171 pulmonary tuberculosis (TB) patients (newly diagnosed 102, 59.6%; retreated 69, 40.3%) were analysed. Drug susceptibility testing was performed using the proportion method and resistant isolates were characterised using the polymerase chain reaction, followed by restriction fragment length polymorphism and/or DNA sequencing, to screen for mutations in rpoB, katG, mabA-inhA and rpsL.
RESULTS: Of the 171 isolates, 16.9% (29/171) were multidrug-resistant. Of the 102 newly diagnosed and 69 retreated cases, respectively 5.9% (6/102) and 33.3% (23/69) were multidrug-resistant. rpoB mutations were found in 100% (31/31) of the RMP-resistant isolates, the most common being S531L in 74.2% (23/31); katG315 mutations were found in 79.6% (35/44) of the INH-resistant isolates; however, no mabA-inhA (-15) mutation was found; rpsL mutations were found in 48.9% (24/49) of the SM-resistant isolates, and codon 43 mutation were found in 42.5% (21/49).
CONCLUSIONS: This study characterises drug resistance-associated mutations in M. tuberculosis, information that could be used for the rapid screening of drug-resistant TB.
Authors:
R Yadav; S Sethi; S K Dhatwalia; D Gupta; A Mewara; M Sharma
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease     Volume:  17     ISSN:  1815-7920     ISO Abbreviation:  Int. J. Tuberc. Lung Dis.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706389     Medline TA:  Int J Tuberc Lung Dis     Country:  France    
Other Details:
Languages:  eng     Pagination:  251-7     Citation Subset:  IM    
Affiliation:
Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
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