| Molecular characterisation of carbohydrate digestion and absorption in equine small intestine. | |
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MedLine Citation:
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PMID: 12117106 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Dietary carbohydrates, when digested and absorbed in the small intestine of the horse, provide a substantial fraction of metabolisable energy. However, if levels in diets exceed the capacity of the equine small intestine to digest and absorb them, they reach the hindgut, cause alterations in microbial populations and the metabolite products and predispose the horse to gastrointestinal diseases. We set out to determine, at the molecular level, the mechanisms, properties and the site of expression of carbohydrate digestive and absorptive functions of the equine small intestinal brush-border membrane. We have demonstrated that the disaccharidases sucrase, lactase and maltase are expressed diversely along the length of the intestine and D-glucose is transported across the equine intestinal brush-border membrane by a high affinity, low capacity, Na+/glucose cotransporter type 1 isoform (SGLT1). The highest rate of transport is in duodenum > jejunum > ileum. We have cloned and sequenced the cDNA encoding equine SGLT1 and alignment with SGLT1 of other species indicates 85-89% homology at the nucleotide and 84-87% identity at the amino acid levels. We have shown that there is a good correlation between levels of functional SGLT1 protein and SGLT1 mRNA abundance along the length of the small intestine. This indicates that the major site of glucose absorption in horses maintained on conventional grass-based diets is in the proximal intestine, and the expression of equine intestinal SGLT1 along the proximal to distal axis of the intestine is regulated at the level of mRNA abundance. The data presented in this paper are the first to provide information on the capacity of the equine intestine to digest and absorb soluble carbohydrates and has implications for a better feed management, pharmaceutical intervention and for dietary supplementation in horses following intestinal resection. |
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Authors:
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J Dyer; E Fernandez-Castaño Merediz; K S H Salmon; C J Proudman; G B Edwards; S P Shirazi-Beechey |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Equine veterinary journal Volume: 34 ISSN: 0425-1644 ISO Abbreviation: Equine Vet. J. Publication Date: 2002 Jul |
Date Detail:
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Created Date: 2002-07-15 Completed Date: 2002-10-22 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0173320 Medline TA: Equine Vet J Country: England |
Other Details:
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Languages: eng Pagination: 349-58 Citation Subset: IM |
Affiliation:
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Department of Veterinary Preclinical Sciences, University of Liverpool, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animal Feed Animal Nutritional Physiological Phenomena Animals Blotting, Northern / veterinary Dietary Carbohydrates / metabolism*, pharmacokinetics Digestion* Disaccharidases / metabolism Gastrointestinal Diseases / diagnosis, veterinary Gastrointestinal Transit Glucose / metabolism Horse Diseases / diagnosis Horses / physiology* Intestinal Absorption* Intestinal Mucosa / enzymology Intestine, Small / metabolism* Membrane Glycoproteins / chemistry, genetics, metabolism Microvilli / enzymology, metabolism Molecular Sequence Data Monosaccharide Transport Proteins / chemistry, genetics, metabolism Nutritive Value RNA, Messenger / metabolism Sequence Alignment / veterinary Sequence Homology, Amino Acid Sodium-Glucose Transporter 1 Species Specificity |
| Chemical | |
Reg. No./Substance:
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0/Dietary Carbohydrates; 0/Membrane Glycoproteins; 0/Monosaccharide Transport Proteins; 0/RNA, Messenger; 0/Sodium-Glucose Transporter 1; 50-99-7/Glucose; EC 3.2.1.-/Disaccharidases |
| Comments/Corrections | |
Comment In:
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Equine Vet J. 2002 Jul;34(4):326-7
[PMID:
12117101
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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