| Molecular characterisation and analysis of bovine rotavirus strains circulating in Ireland 2002-2004. | |
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MedLine Citation:
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PMID: 16844325 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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One hundred and two faecal samples were collected from calves diagnosed with rotavirus infection, in the southern region of Ireland, from 2002 to 2004. Ninety one percent (n=93) were confirmed positive for rotavirus, using latex agglutination and enzyme linked immunosorbent assay (ELISA) methods. Determination of the G- and P-types was carried out using nested reverse transcriptase polymerase chain reaction (nRT-PCR). G6 was the most prevalent genotype, accounting for 80.6% (75/93), G10 accounted for 6.5% (6/93) and G6G10 mixed types accounted for 9.7% (9/93) of the collection. Rotavirus in three of the samples (3.2%) could not be characterised with any of the five G-specific primers used in this study. A subset of the positive samples (n=54) was examined for their P-type specificities, P[5] and P[11] accounted for 77.8% (42/54), and 9.3% (5/54), respectively. One P[1] genotype (1.9%) was found in the collection. P[5] and P[11] mixed genotypes accounted for 11% (6/54) of the study. The genotypes corresponded to the UK-like strain (G6P[5]) 57.4%, KN4-like strain (G6[P11]) 7.4%, B223-like strain (G10P[11]) and NCDV-like strain (G6P[1]) 1.9% each. The unusual combination of G10P[5] accounted for 7.4%, with mixed infections G6+G10P[5] and G6P[5]+P[11] representing 13% and 11%, respectively. This is the first time that the G- and P-types of bovine rotaviruses (BRVs) have been determined in Ireland, and this study contributes to a better understanding of the epidemiology of such viruses circulating in Ireland. |
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Authors:
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N Reidy; G Lennon; S Fanning; E Power; H O'Shea |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-05-16 |
Journal Detail:
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Title: Veterinary microbiology Volume: 117 ISSN: 0378-1135 ISO Abbreviation: Vet. Microbiol. Publication Date: 2006 Oct |
Date Detail:
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Created Date: 2006-09-25 Completed Date: 2007-09-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7705469 Medline TA: Vet Microbiol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 242-7 Citation Subset: IM |
Affiliation:
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Virology Unit, Department of Biological Sciences, Cork Institute of Technology, Bishopstown, Cork, Ireland. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AY629556; AY629557; AY644385 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Cattle Cattle Diseases / epidemiology* Enzyme-Linked Immunosorbent Assay / methods, veterinary Feces / microbiology Genotype Ireland / epidemiology Latex Fixation Tests / methods, veterinary Molecular Sequence Data Phylogeny RNA, Viral / chemistry Rotavirus / classification, genetics, isolation & purification* Rotavirus Infections / epidemiology, veterinary* |
| Chemical | |
Reg. No./Substance:
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0/RNA, Viral |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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