Document Detail


Molecular changes of preclinical scrapie can be detected by infrared spectroscopy.
MedLine Citation:
PMID:  11943801     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Infrared (IR) microspectroscopy was used to detect disease-associated molecular changes spatially resolved in cryosections of scrapie-infected tissue of the CNS. The results show that IR spectra can be used for the discrimination between normal and 263K scrapie-infected hamster nervous tissue not only in the terminal stage of the disease but also in early clinical and even in the preclinical stage at 90 d after oral infection. The nuclei of the cranial nerves located in the medulla oblongata were especially well suited for an early detection of the diseased state by IR microspectroscopy. The most prominent molecular changes indicated by the IR spectra were located between 1300 and 1000 cm(-1), a region that contains contributions primarily from carbohydrates and the phosphate backbones of nucleic acids but also from membrane constituents.
Authors:
Janina Kneipp; Michael Beekes; Peter Lasch; Dieter Naumann
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of neuroscience : the official journal of the Society for Neuroscience     Volume:  22     ISSN:  1529-2401     ISO Abbreviation:  J. Neurosci.     Publication Date:  2002 Apr 
Date Detail:
Created Date:  2002-04-10     Completed Date:  2002-04-22     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  8102140     Medline TA:  J Neurosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2989-97     Citation Subset:  IM    
Affiliation:
PG3, Robert Koch-Institut, D-13353 Berlin, Germany. janina.kneipp@epost.de
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MeSH Terms
Descriptor/Qualifier:
Administration, Oral
Animals
Cerebellar Nuclei / pathology
Cerebellum / pathology*
Cluster Analysis
Cricetinae
Disease Models, Animal
Disease Progression
Female
Hypoglossal Nerve / pathology
Immunohistochemistry
Medulla Oblongata / pathology*
Mesocricetus
Microscopy / instrumentation,  methods*
Multivariate Analysis
Neurons / pathology
PrPSc Proteins / administration & dosage,  isolation & purification,  pathogenicity
Predictive Value of Tests
Reproducibility of Results
Scrapie / diagnosis*,  pathology,  transmission
Solitary Nucleus / pathology
Spectroscopy, Fourier Transform Infrared*
Vagus Nerve / pathology
Chemical
Reg. No./Substance:
0/PrPSc Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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