Document Detail


Molecular breeding of transgenic rice plants expressing a bacterial chlorocatechol dioxygenase gene.
MedLine Citation:
PMID:  12147507     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cbnA gene encoding the chlorocatechol dioxygenase gene from Ralstonia eutropha NH9 was introduced into rice plants. The cbnA gene was expressed in transgenic rice plants under the control of a modified cauliflower mosaic virus 35S promoter. Western blot analysis using anti-CbnA protein indicated that the cbnA gene was expressed in leaf tissue, roots, culms, and seeds. Transgenic rice calluses expressing the cbnA gene converted 3-chlorocatechol to 2-chloromucote efficiently. Growth and morphology of the transgenic rice plants expressing the cbnA gene were not distinguished from those of control rice plants harboring only a Ti binary vector. It is thus possible to breed transgenic plants that degrade chloroaromatic compounds in soil and surface water.
Authors:
Masami Shimizu; Tetsuya Kimura; Takayoshi Koyama; Katsuhisa Suzuki; Naoto Ogawa; Kiyotaka Miyashita; Kazuo Sakka; Kunio Ohmiya
Related Documents :
17390797 - Study of effects of bt maize (zea mays) events on lepidoptera ostrinia nubilalis, sesam...
10073237 - Characterization of phenotype resistance to plum pox of transgenic plums expressing plu...
17149627 - Growth promoting effect of a transgenic bacillus mucilaginosus on tobacco planting.
10209137 - Production of new/modified proteins in transgenic plants.
848957 - Microbiological profiles of the viking spacecraft.
24530547 - Increased pond depth improves algal productivity and nutrient removal in wastewater tre...
Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  68     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-07-30     Completed Date:  2002-10-17     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4061-6     Citation Subset:  IM    
Affiliation:
Faculty of Bioresources, Mie University, Tsu 514-8507, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Biodegradation, Environmental
Breeding / methods*
Catechols / metabolism
Cupriavidus necator / enzymology,  genetics
Dioxygenases*
Oryza sativa / genetics*,  metabolism,  physiology
Oxygenases / genetics*,  metabolism*
Plants, Genetically Modified / genetics,  metabolism*,  physiology
Transformation, Genetic
Chemical
Reg. No./Substance:
0/Catechols; 4018-65-9/3-chlorocatechol; EC 1.13.-/Oxygenases; EC 1.13.11.-/Dioxygenases; EC 1.13.11.-/catechol oxygenase II
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Ability of nonpathogenic Fusarium oxysporum strain Fo47 to induce resistance against Pythium ultimum...
Next Document:  Exploration of inorganic C and N assimilation by soil microbes with time-of-flight secondary ion mas...