Document Detail

Molecular breeding of transgenic rice plants expressing a bacterial chlorocatechol dioxygenase gene.
MedLine Citation:
PMID:  12147507     Owner:  NLM     Status:  MEDLINE    
The cbnA gene encoding the chlorocatechol dioxygenase gene from Ralstonia eutropha NH9 was introduced into rice plants. The cbnA gene was expressed in transgenic rice plants under the control of a modified cauliflower mosaic virus 35S promoter. Western blot analysis using anti-CbnA protein indicated that the cbnA gene was expressed in leaf tissue, roots, culms, and seeds. Transgenic rice calluses expressing the cbnA gene converted 3-chlorocatechol to 2-chloromucote efficiently. Growth and morphology of the transgenic rice plants expressing the cbnA gene were not distinguished from those of control rice plants harboring only a Ti binary vector. It is thus possible to breed transgenic plants that degrade chloroaromatic compounds in soil and surface water.
Masami Shimizu; Tetsuya Kimura; Takayoshi Koyama; Katsuhisa Suzuki; Naoto Ogawa; Kiyotaka Miyashita; Kazuo Sakka; Kunio Ohmiya
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  68     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-07-30     Completed Date:  2002-10-17     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4061-6     Citation Subset:  IM    
Faculty of Bioresources, Mie University, Tsu 514-8507, Japan.
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MeSH Terms
Biodegradation, Environmental
Breeding / methods*
Catechols / metabolism
Cupriavidus necator / enzymology,  genetics
Oryza sativa / genetics*,  metabolism,  physiology
Oxygenases / genetics*,  metabolism*
Plants, Genetically Modified / genetics,  metabolism*,  physiology
Transformation, Genetic
Reg. No./Substance:
0/Catechols; 4018-65-9/3-chlorocatechol; EC 1.13.-/Oxygenases; EC 1.13.11.-/Dioxygenases; EC 1.13.11.-/catechol oxygenase II

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