Document Detail


Molecular biology of phase-variable lipopolysaccharide biosynthesis by Haemophilus influenzae.
MedLine Citation:
PMID:  1588188     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Several chromosomal loci are involved in lipopolysaccharide (LPS) biosynthesis by Haemophilus influenzae. Two of these, lic1 and lic2, contain multiple open-reading frames (ORFs) and include tandem repeats of the tetramer CAAT within and at the 5' end of the coding region of the first ORF in each locus. Variation in the number of repeats of CAAT is involved in the variable expression of LPS epitopes, and genes within these loci are involved in the biosynthesis of these epitopes. lic3 also contains multiple ORFs and the CAAT repeats in the same arrangement as in the other two lic loci. However, in lic3 metabolic functions are encoded by the downstream genes. ORF2 is galE, encoding uridine 5'-diphosphogalactose 4-epimerase, and ORF4 is adk, encoding adenylate kinase. A mutant H. influenzae with a deleted galE gene had an altered LPS when grown on media lacking galactose and was of reduced virulence in infant rats.
Authors:
D J Maskell; M J Szabo; P D Butler; A E Williams; E R Moxon
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of infectious diseases     Volume:  165 Suppl 1     ISSN:  0022-1899     ISO Abbreviation:  J. Infect. Dis.     Publication Date:  1992 Jun 
Date Detail:
Created Date:  1992-06-25     Completed Date:  1992-06-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0413675     Medline TA:  J Infect Dis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  S90-2     Citation Subset:  AIM; IM    
Affiliation:
Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
DNA, Bacterial / chemistry
Gene Expression Regulation, Bacterial*
Haemophilus Infections / microbiology*
Haemophilus influenzae / genetics*,  metabolism,  pathogenicity
Lipopolysaccharides / biosynthesis*,  genetics
Mutagenesis, Insertional
Open Reading Frames
Rats
Virulence / genetics
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 0/Lipopolysaccharides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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