Document Detail


Molecular and biological aspects of early germ cell development in interspecies hybrids between chickens and pheasants.
MedLine Citation:
PMID:  21111472     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Interspecific hybrids provide insights into fundamental genetic principles, and may prove useful for biotechnological applications and as tools for the conservation of endangered species. In the present study, interspecies hybrids were generated between the Korean ring-necked pheasant (Phasianus colchicus) and the White Leghorn chicken (Gallus gallus domesticus). We determined whether these hybrids were good recipients for the production of germline chimeric birds. PCR-based species-specific amplification and karyotype analyses showed that the hybrids inherited genetic material from both parents. Evaluation of biological function indicated that the growth rates of hybrids during the exponential phase (body weight/week) were similar to those of the pheasant but not the chicken, and that the incubation period for hatching was significantly different from that of both parents. Primordial germ cells (PGCs) of hybrids reacted with a pheasant PGC-specific antibody and circulated normally in blood vessels. The peak time of hybrid PGC migration was equivalent to that of the pheasant. In late embryonic stages, germ cells were detected by the QCR1 antibody on 15 d male gonads and were normally localized in the seminiferous cords. We examined the migration ability and developmental localization of exogenous PGCs transferred into the blood vessels of 63 h hybrid embryos. Donor-derived PGCs reacted with a donor-specific antibody were detected on 7 d gonads and the seminiferous tubules of hatchlings. Therefore, germ cell transfer into developing embryos of an interspecies hybrid can be efficiently used for the conservation of threatened animals and endangered species, and many biotechnological applications.
Authors:
Seok Jin Kang; Sea Hwan Sohn; Kyung Soo Kang; Hyung Chul Lee; Seul Ki Lee; Jin Won Choi; Jae Yong Han
Publication Detail:
Type:  Journal Article     Date:  2010-11-26
Journal Detail:
Title:  Theriogenology     Volume:  75     ISSN:  1879-3231     ISO Abbreviation:  Theriogenology     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-02-07     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  696-706     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Inc. All rights reserved.
Affiliation:
WCU Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea; Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Korea.
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