Document Detail

Molecular and biochemical characterization of a protein cyclophilin from the nematode Haemonchus contortus( P ).
MedLine Citation:
PMID:  15830208     Owner:  NLM     Status:  MEDLINE    
We have cloned, sequenced and expressed a gene of Haemonchus contortus that encodes a protein (termed HcCYP) consisting of a cyclophilin domain and an RNA recognition motif (RRM). An antiserum raised against the recombinant protein showed that HcCYP was present in the insoluble fraction (mostly nuclear) of the parasite homogenate. The recombinant protein possessed the typical cis-trans peptidyl-prolyl isomerase activity of cyclophilins and this activity was inhibited by the immunosuppressant cyclosporin A. The N-terminal portion of the molecule, carrying the RRM, was able to bind to nucleic acids, whereas the C-terminal portion did not have any binding activity. The possible function of HcCYP in the parasite is discussed on the basis of information available on similar proteins in other organisms.
Cristiana Valle; Anna Rita Troiani; Patrizio Lazzaretti; Jacques Bouvier; Donato Cioli; Mo-Quen Klinkert
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Publication Detail:
Type:  Journal Article     Date:  2005-04-14
Journal Detail:
Title:  Parasitology research     Volume:  96     ISSN:  0932-0113     ISO Abbreviation:  Parasitol. Res.     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-07-01     Completed Date:  2005-10-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8703571     Medline TA:  Parasitol Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  199-205     Citation Subset:  IM    
Institute for Cell Biology Italian National Research Council, Via Ramarini 32, 00016 Monterotondo Scalo, Italy.
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MeSH Terms
Amino Acid Motifs
Amino Acid Sequence
Cloning, Molecular
Cyclophilins / genetics*,  metabolism
DNA, Helminth*
Genes, Helminth / genetics*
Haemonchus / chemistry,  genetics*
Molecular Sequence Data
Peptidylprolyl Isomerase / metabolism
RNA, Helminth
Recombinant Proteins / genetics,  metabolism
Reg. No./Substance:
0/DNA, Helminth; 0/RNA, Helminth; 0/Recombinant Proteins; EC 5.2.1.-/Cyclophilins; EC Isomerase

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