Document Detail


Molecular and biochemical characterization of AtPAP15, a purple acid phosphatase with phytase activity, in Arabidopsis.
MedLine Citation:
PMID:  19633233     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Purple acid phosphatase (PAP) catalyzes the hydrolysis of phosphate monoesters and anhydrides to release phosphate within an acidic pH range. Among the 29 PAP-like proteins in Arabidopsis (Arabidopsis thaliana), AtPAP15 (At3g07130) displays a greater degree of amino acid identity with soybean (Glycine max; GmPHY) and tobacco (Nicotiana tabacum) PAP (NtPAP) with phytase activity than the other AtPAPs. In this study, transgenic Arabidopsis that expressed an AtPAP15 promoterbeta-glucuronidase (GUS) fusion protein showed that AtPAP15 expression was developmentally and temporally regulated, with strong GUS staining at the early stages of seedling growth and pollen germination. The expression was also organ/tissue specific, with strongest GUS staining in the vasculature, pollen grains, and roots. The recombinant AtPAP purified from transgenic tobacco exhibited broad substrate specificity with moderate phytase activity. AtPAP15 T-DNA insertion lines exhibited a lower phytase and phosphatase activity in seedling and germinating pollen and lower pollen germination rate compared with the wild type and their complementation lines. Therefore, AtPAP15 likely mobilizes phosphorus reserves in plants, particularly during seed and pollen germination. Since AtPAP15 is not expressed in the root hair or in the epidermal cells, it is unlikely to play any role in external phosphorus assimilation.
Authors:
Ruibin Kuang; Kam-Ho Chan; Edward Yeung; Boon Leong Lim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-07-24
Journal Detail:
Title:  Plant physiology     Volume:  151     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-09-03     Completed Date:  2009-11-23     Revised Date:  2010-09-27    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  199-209     Citation Subset:  IM    
Affiliation:
School of Biological Sciences, University of Hong Kong, Pokfulam, Hong Kong, China.
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MeSH Terms
Descriptor/Qualifier:
Acid Phosphatase / genetics,  metabolism*
Arabidopsis / enzymology*
Arabidopsis Proteins / genetics,  metabolism*
Cloning, Molecular
DNA, Bacterial / genetics
Gene Expression Regulation, Enzymologic / physiology
Gene Expression Regulation, Plant / physiology*
Genetic Complementation Test
Multienzyme Complexes / genetics,  metabolism*
Mutagenesis, Insertional
Mutation
Plants, Genetically Modified
Substrate Specificity
Tobacco / genetics
Chemical
Reg. No./Substance:
0/Arabidopsis Proteins; 0/DNA, Bacterial; 0/Multienzyme Complexes; 0/T-DNA; EC 3.1.3.-/PAP15 protein, Arabidopsis; EC 3.1.3.2/Acid Phosphatase
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