| Molecular and biochemical analysis of ceruloplasmin expression in rabbit and rat ciliary body. | |
MedLine Citation:
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PMID: 16500766 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To verify the capability of rabbit and rat ciliary body to synthesize and secrete ceruloplasmin. METHODS: Isolated ciliary body (CB) was cultured in the presence of [35S]-methionine, and the incubation medium was processed for immunoprecipitation. Total RNA from CB was processed for RT-PCR, and the amplification products were sequenced. Also, sections of CB were immunostained for the localization of ceruloplasmin. RESULTS: A labeled peptide, having a molecular weight of about 135 kDa, the expected size of ceruloplasmin, was immunopurified in the incubation media from both animal species. The RT-PCR and sequencing experiments detected the presence of ceruloplasmin mRNA in rat samples. Both layers of rabbit and rat ciliary epithelium (CE) exhibited ceruloplasmin reactivity after immunohistochemical processing. CONCLUSIONS: Taken altogether, these results indicate the CB, particularly its epithelium, as one of the possible sources of the ocular intrinsic ceruloplasmin. |
Authors:
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Rubens Bertazolli-Filho; Eduardo Miguel Laicine; Antonio Haddad; Maria Laura Pinto Rodrigues |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Current eye research Volume: 31 ISSN: 0271-3683 ISO Abbreviation: Curr. Eye Res. Publication Date: 2006 Feb |
Date Detail:
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Created Date: 2006-02-27 Completed Date: 2006-04-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8104312 Medline TA: Curr Eye Res Country: England |
Other Details:
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Languages: eng Pagination: 155-61 Citation Subset: IM |
Affiliation:
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Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos, Faculdade de Medicina de Ribeirão Preto, USP, Ribeirão Preto, Brazil. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Ceruloplasmin / genetics*, metabolism* Ciliary Body / enzymology* Immunoenzyme Techniques Immunoprecipitation Male Organ Culture Techniques RNA, Messenger / metabolism Rabbits Rats Reverse Transcriptase Polymerase Chain Reaction Transferrin / genetics |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 11096-37-0/Transferrin; EC 1.16.3.1/Ceruloplasmin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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