Document Detail


Molecular basis for the presence of glycosylated onco-foetal fibronectin in oral carcinomas: the production of glycosylated onco-foetal fibronectin by carcinoma cells.
MedLine Citation:
PMID:  16857413     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Glycosylated onco-foetal fibronectin (GOF) deposited in the stroma of oral squamous cell carcinomas correlates with survival. One of the two polypeptide GalNAc-transferases, GalNAc-T3 or GalNAc-T6, is required for the biosynthesis of GOF by the initiation of a unique O-glycan in the alternative spliced IIICS region. Using cell culture experiments, immunohistochemical staining of primary tissue, and RT-PCR of tumour cells isolated by laser capture techniques we have examined the molecular basis for the production of GOF in oral carcinomas. Immuno-histochemical investigation confirmed the stromal deposition of GOF in oral carcinomas. However, neither GalNAc-T3 nor GalNAc-T6 could be detected in stromal fibroblasts. In contrast both transferases were present in the oral squamous carcinoma cells, suggesting that GOF is produced by the oral cancer cells and not only the stromal cells. RT-PCR analysis of RNA isolated from carcinoma cells provided further support for this conclusion by demonstrating in-splicing of the alternative spliced IIICS domain in GOF.
Authors:
Hans H Wandall; Sally Dabelsteen; Jens Ahm Sørensen; Annelise Krogdahl; Ulla Mandel; Erik Dabelsteen
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Publication Detail:
Type:  Journal Article     Date:  2006-07-20
Journal Detail:
Title:  Oral oncology     Volume:  43     ISSN:  1368-8375     ISO Abbreviation:  Oral Oncol.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-02-16     Completed Date:  2007-11-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9709118     Medline TA:  Oral Oncol     Country:  England    
Other Details:
Languages:  eng     Pagination:  301-9     Citation Subset:  IM    
Affiliation:
Department of Oral Diagnostics, The Faculty of Health Sciences, University of Copenhagen, Nørre Alle 20, DK-2200 Copenhagen N, Denmark.
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MeSH Terms
Descriptor/Qualifier:
Actins / analysis
Biocompatible Materials
Carcinoma, Squamous Cell / enzymology,  genetics*,  pathology
Cell Line, Tumor
Cells, Cultured
Collagen
Drug Combinations
Fibroblasts / pathology
Fibronectins / analysis*
Glycosylation
Humans
Immunohistochemistry / methods
Keratinocytes / pathology
Laminin
Mouth Neoplasms / enzymology,  genetics*,  pathology
Muscle, Smooth / pathology
Proteoglycans
Sialyltransferases / analysis
Chemical
Reg. No./Substance:
0/Actins; 0/Biocompatible Materials; 0/Drug Combinations; 0/Fibronectins; 0/Laminin; 0/Proteoglycans; 0/oncofetal fibronectin; 119978-18-6/matrigel; 9007-34-5/Collagen; EC 2.4.99.-/GalNAc sialyltransferase VI; EC 2.4.99.-/Neu5Ac N-acetylgalactosamine 2,6-sialyltransferase; EC 2.4.99.-/Sialyltransferases

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