| Molecular authentication of the Oriental medicines Pericarpium Citri Reticulatae and Citri Unshius Pericarpium using SNP markers. | |
| | |
MedLine Citation:
|
PMID: 22173103 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Pericarpium Citri Reticulatae and Citri Unshius Pericarpium are important Oriental medicinal materials used in many prescriptions. Among the Citrus species, the dried peels of C. japonica, C. maxima, and C. trifoliata are found to be adulterants and substitutes of Pericarpium Citri Reticulatae and Citri Unshius Pericarpium. In order to develop a simple and reliable DNA method for authentication of these two medicinal materials, nuclear ribosomal internal transcribed spacer (ITS) region was targeted for molecular analysis. A host of single nucleotide polymorphism (SNP) sites were detected among ITS sequences of five Citrus species. From two SNP sites, two modified specific primers were designed for authentication of Pericarpium Citri Reticulatae and Citri Unshius Pericarpium using multiplex PCR. The established multiplex allele-specific PCR system was proven to be effective for simultaneous authentication of Pericarpium Citri Reticulatae and Citri Unshius Pericarpium. The scheme used in this study could be adapted for determination of the botanical identity and origin of other medicinal materials. |
| | |
Authors:
|
Hongtao Wang; Min-Kyeoung Kim; Hee-Neoung Lee; Haizhu Jin; Jiayu Chen; Deok-Chun Yang |
Related Documents
:
|
21968823 - The u.s. nrc and the adr process learning experiences. 5842593 - Severe tetanus: its complications and management. 12171353 - Opportunities for sustainable riverine management in the queensland murray-darling basin. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-11-25 |
Journal Detail:
|
Title: Gene Volume: - ISSN: 1879-0038 ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
|
Created Date: 2011-12-16 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7706761 Medline TA: Gene Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011. Published by Elsevier B.V. |
Affiliation:
|
College of Life Science, Yantai University, Yantai, 264005, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Current Match Analysis Techniques Can Underestimate Intense Periods of High-Velocity Running.
Next Document: Bisphenol A and phthalates exhibit similar toxicogenomics and health effects.