Document Detail

Molecular assessment of post-BMT chimerism using various biologic specimens and automated DNA sizing technology.
MedLine Citation:
PMID:  10813540     Owner:  NLM     Status:  MEDLINE    
Highly polymorphic microsatellite markers provide useful genetic markers for detection of complete or mixed chimerism in patients after allogeneic BMT (allo-BMT). We report application of automated DNA sizing technology for detection of post-BMT chimerism using fresh peripheral blood, BM, or archival blood smears and various DNA isolation techniques. Donors' and recipients' DNA was amplified with fluorescent PCR primers specific for short tandem repeat (STR) marker loci: FGA, VWA, TH01, F13A1, D21S11. Chimerism was assessed in 14 recipients after allo-BMT. A complete chimerism was detected in 10 patients, in 3 patients we observed fluctuations of chimerism status, and mixed chimerism was assessed in 1 patient. We show that DNA from different types of biologic specimens (whole peripheral blood, BM suspension, archival blood smears), prepared according to the various isolation techniques (salting-out method, phenol chloroform extraction, Chelex procedure) and amplified with fluorescent PCR primers for microsatellite markers, enable identification of chimerism status following allo-BMT in children.
J Jółkowska; J Wachowiak; A Lange; M Kwissa; M Witt
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of hematotherapy & stem cell research     Volume:  9     ISSN:  1525-8165     ISO Abbreviation:  J. Hematother. Stem Cell Res.     Publication Date:  2000 Apr 
Date Detail:
Created Date:  2000-08-21     Completed Date:  2000-08-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100892915     Medline TA:  J Hematother Stem Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  263-8     Citation Subset:  IM    
Institute of Human Genetics, Polish Academy of Sciences, Poznań.
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MeSH Terms
Anemia, Aplastic / genetics,  therapy
Bone Marrow Cells
Bone Marrow Transplantation*
Child, Preschool
DNA / analysis,  blood
DNA, Satellite
Genetic Markers
Leukemia / genetics,  therapy
Molecular Weight
Polymerase Chain Reaction
Severe Combined Immunodeficiency / genetics,  therapy
Transplantation Chimera / genetics*
Transplantation, Homologous
Reg. No./Substance:
0/DNA, Satellite; 0/Genetic Markers; 9007-49-2/DNA

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