Document Detail


Molecular aspects of human FcgammaR interactions with IgG: functional and therapeutic consequences.
MedLine Citation:
PMID:  16797726     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The binding of IgG antibodies to receptors for the Fc region of IgG (FcgammaR) is a critical step for the initiation and/or the control of effector immune functions once immune complexes have been formed. Site-directed and random mutagenesis as well as domain-swapping, NMR and X-ray cristallography have made it possible to get detailed insights in the molecular mechanisms that govern IgG/FcgammaR interactions and to define some of the structural determinants that impact IgG binding to the various FcgammaR. It has demonstrated the role of particular stretches and individual residues located in the lower hinge region of the CH2 domain and in the CH2 and CH3 domains of the Fc region. The importance of the sugar components linked to asparagine 297 in the binding properties of IgG1, the human IgG isotype the most widely used in antibody-based therapies, has been also highlighted. These data have led to the engineering of a new generation of monoclonal antibodies for therapeutic use with optimized effector functions.
Authors:
Sophie Sibéril; Charles-Antoine Dutertre; Charlotte Boix; Emmanuelle Bonnin; Renée Ménez; Enrico Stura; Sylvie Jorieux; Wolf-Herman Fridman; Jean-Luc Teillaud
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review     Date:  2006-06-12
Journal Detail:
Title:  Immunology letters     Volume:  106     ISSN:  0165-2478     ISO Abbreviation:  Immunol. Lett.     Publication Date:  2006 Aug 
Date Detail:
Created Date:  2006-08-21     Completed Date:  2006-10-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7910006     Medline TA:  Immunol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  111-8     Citation Subset:  IM    
Affiliation:
Unité INSERM 255, IFR58, Université René Descartes-Paris 5, Université Pierre et Marie Curie-Paris 6, Centre de Recherches Biomédicales des Cordeliers, Paris, France.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal / genetics,  immunology*,  therapeutic use
Humans
Immunoglobulin Fc Fragments / genetics,  immunology*,  therapeutic use
Immunoglobulin G / genetics,  immunology*,  therapeutic use
Mutagenesis, Site-Directed / methods
Protein Binding / genetics,  immunology
Receptors, Fc / genetics,  immunology*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Immunoglobulin Fc Fragments; 0/Immunoglobulin G; 0/Receptors, Fc

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