Document Detail

Molecular aspects of chloride cell formation in Oncorhynchus. II. Isolation and characterization of gill transfer RNA's during active salt secretion.
MedLine Citation:
PMID:  850119     Owner:  NLM     Status:  MEDLINE    
The transfer ribonucleic acids (tRNAs) from gill filaments of coho salmon, Oncorynchus kisutch, have been isolated and were separated into individual species of acceptor tRNAs by reversed-phase column chromotography. Crude tRNAs isolated from gill filaments undergoing active salt excretion were acrylated with 14C-amino acids and combined with tRNAs acylated with 3H-amino acids, but obtained from non-secreting filaments. The radioactively labeled tRNA mixture was cochromatographed and the results showed significant differences in the elution profiles of tRNAasp and tRNAtyr. These elution profiles may reflect the differentiation of the chloride cell population that is found within the gill epithelium.
F P Conte
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of experimental zoology     Volume:  199     ISSN:  0022-104X     ISO Abbreviation:  J. Exp. Zool.     Publication Date:  1977 Mar 
Date Detail:
Created Date:  1977-05-27     Completed Date:  1977-05-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375365     Medline TA:  J Exp Zool     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  395-402     Citation Subset:  IM    
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MeSH Terms
Amino Acids / metabolism
Aspartic Acid / metabolism
Cell Differentiation
Chlorides / metabolism*
Epithelial Cells
Epithelium / metabolism
Gills / metabolism*
RNA, Transfer / metabolism*
Salmon / metabolism*
Serine / metabolism
Sodium Chloride / secretion*
Tyrosine / metabolism
Reg. No./Substance:
0/Amino Acids; 0/Chlorides; 55520-40-6/Tyrosine; 56-45-1/Serine; 56-84-8/Aspartic Acid; 7647-14-5/Sodium Chloride; 9014-25-9/RNA, Transfer

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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