Document Detail


Molecular architecture of fungal cell walls. An approach by use of fluorescent markers.
MedLine Citation:
PMID:  11596507     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fluorescent probes have been applied to analyze the molecular architecture of fungal cell surfaces. Binding patterns of aniline blue and FITC-labeled wheat germ agglutinin (FITC-WGA) elucidated class-specific properties. Aniline-blue-induced fluorescence was distributed over the entire cell walls from Ascomycetes, but was confined to sporangial walls of Zygomocetes, hyphal tips and a few other sites in Basidiomycetes, while no fluorescence was found with sporangia and rhizoids of Chytridiales. FITC-WGA in Zygomycetes and in Ascomycetes was restricted to few sites (e.g. apex of hyphae), in Basidiomycetes and Chytridiales label was evenly associated with the entire surface of hyphal walls, or sporangia and rhizoids. For Oomycetes, Zygomycetes, Ascomycetes, and Basidiomycetes differences in the molecular architecture between apex and hyphal side walls were discerned, although the chemical nature of these differences is distinct for each class. Species specific differences, due to differences in binding patterns of several lectins are not apparent at fungal cell surfaces. The degree of intraspecies variation was found to be larger than interspecies diversification, suggesting changeableness of the molecular architecture of fungal cell walls. This is in contrast to assertions which we made by working on algae. There species-specific lectin binding patterns have been described.
Authors:
P von Sengbusch; J Hechler; U Müller
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of cell biology     Volume:  30     ISSN:  0171-9335     ISO Abbreviation:  Eur. J. Cell Biol.     Publication Date:  1983 May 
Date Detail:
Created Date:  2001-10-12     Completed Date:  2001-11-01     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7906240     Medline TA:  Eur J Cell Biol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  305-12     Citation Subset:  IM    
Affiliation:
Institut für Allgemeine Botanik und Botanischer Garten, Universität Hamburg/Bundesrepublik Deutschland.
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MeSH Terms
Descriptor/Qualifier:
Ascomycota / ultrastructure
Basidiomycota / ultrastructure
Benzenesulfonates / pharmacology
Cell Wall / pathology*,  ultrastructure*
Chytridiomycota / ultrastructure
Fluorescein-5-isothiocyanate / pharmacology
Fluorescent Dyes / pharmacology
Fungi / cytology*,  ultrastructure
Lectins / chemistry
Microscopy, Fluorescence / methods*
Species Specificity
Wheat Germ Agglutinins / pharmacology
Chemical
Reg. No./Substance:
0/Benzenesulfonates; 0/Fluorescent Dyes; 0/Lectins; 0/Wheat Germ Agglutinins; 3326-32-7/Fluorescein-5-isothiocyanate; 4404-43-7/C.I. Fluorescent Brightening Agent 28

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