Document Detail


Molecular architecture and assembly principles of Vibrio cholerae biofilms.
MedLine Citation:
PMID:  22798614     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In their natural environment, microbes organize into communities held together by an extracellular matrix composed of polysaccharides and proteins. We developed an in vivo labeling strategy to allow the extracellular matrix of developing biofilms to be visualized with conventional and superresolution light microscopy. Vibrio cholerae biofilms displayed three distinct levels of spatial organization: cells, clusters of cells, and collections of clusters. Multiresolution imaging of living V. cholerae biofilms revealed the complementary architectural roles of the four essential matrix constituents: RbmA provided cell-cell adhesion; Bap1 allowed the developing biofilm to adhere to surfaces; and heterogeneous mixtures of Vibrio polysaccharide, RbmC, and Bap1 formed dynamic, flexible, and ordered envelopes that encased the cell clusters.
Authors:
Veysel Berk; Jiunn C N Fong; Graham T Dempsey; Omer N Develioglu; Xiaowei Zhuang; Jan Liphardt; Fitnat H Yildiz; Steven Chu
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Science (New York, N.Y.)     Volume:  337     ISSN:  1095-9203     ISO Abbreviation:  Science     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-07-16     Completed Date:  2012-07-30     Revised Date:  2013-07-17    
Medline Journal Info:
Nlm Unique ID:  0404511     Medline TA:  Science     Country:  United States    
Other Details:
Languages:  eng     Pagination:  236-9     Citation Subset:  IM    
Affiliation:
Department of Physics, University of California, Berkeley, CA 94720, USA. vberk@berkeley.edu
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MeSH Terms
Descriptor/Qualifier:
Bacterial Adhesion
Bacterial Proteins / analysis*,  genetics,  metabolism
Biofilms / growth & development*
Cell Membrane / chemistry
Polysaccharides, Bacterial / metabolism
Vibrio cholerae O1 / chemistry*,  cytology,  physiology*
Grant Support
ID/Acronym/Agency:
AI055987/AI/NIAID NIH HHS; GM068518/GM/NIGMS NIH HHS; GM096450/GM/NIGMS NIH HHS; R01 AI055987/AI/NIAID NIH HHS; R01 GM096450/GM/NIGMS NIH HHS; //Howard Hughes Medical Institute
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Polysaccharides, Bacterial
Comments/Corrections
Comment In:
Nat Rev Microbiol. 2012 Sep;10(9):597   [PMID:  22842660 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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