Document Detail

Molecular analysis of a sulphate-reducing consortium used to treat metal-containing effluents.
MedLine Citation:
PMID:  16946985     Owner:  NLM     Status:  MEDLINE    
A sulphate-reducing consortium used in a bioprocess to remove toxic metals from solution as insoluble sulphides, was characterised using molecular (PCR-based) and traditional culturing techniques. After prolonged cultivation under anoxic biofilm-forming conditions, the mixed culture contained a low diversity of sulphate-reducing bacteria, dominated by one strain closely related to Desulfomicrobium norvegicum, identified by three independent PCR-based analyses. The genetic targets used were the 16S rRNA gene, the 16S-23S rRNA gene intergenic spacer region and the disulfite reductase (dsr) gene, which is conserved amongst all known sulphate-reducing bacteria. This organism was also isolated by conventional anaerobic techniques, confirming its presence in the mixed culture. A surprising diversity of other non-sulphate-reducing facultative and obligate anaerobes were detected, supporting a model of the symbiotic/commensal nature of carbon and energy fluxes in such a mixed culture while suggesting the physiological capacity for a wide range of biotransformations by this stable microbial consortium.
C Boothman; S Hockin; D E Holmes; G M Gadd; J R Lloyd
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-08-31
Journal Detail:
Title:  Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine     Volume:  19     ISSN:  0966-0844     ISO Abbreviation:  Biometals     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2006-11-03     Completed Date:  2007-02-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9208478     Medline TA:  Biometals     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  601-9     Citation Subset:  IM    
School of Earth, Atmospheric and Environmental Sciences and Williamson Research Centre for Molecular Environmental Science, University of Manchester, Manchester , UK.
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MeSH Terms
Bacteria / genetics*,  metabolism
Biodegradation, Environmental
Bioreactors / microbiology
Deltaproteobacteria / genetics,  isolation & purification,  metabolism
Metals / metabolism*
Polymerase Chain Reaction
RNA, Ribosomal, 16S / genetics
Sulfates / metabolism*
Water Microbiology
Water Pollutants, Chemical / metabolism*
Reg. No./Substance:
0/Metals; 0/RNA, Ribosomal, 16S; 0/Sulfates; 0/Water Pollutants, Chemical

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