| Molecular analysis of sucrose metabolism of Erwinia amylovora and influence on bacterial virulence. | |
| | |
MedLine Citation:
|
PMID: 10986236 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Sucrose is an important storage and transport sugar of plants and an energy source for many phytopathogenic bacteria. To analyze regulation and biochemistry of sucrose metabolism of the fire blight pathogen Erwinia amylovora, a chromosomal fragment which enabled Escherichia coli to utilize sucrose as sole carbon source was cloned. By transposon mutagenesis, the scr regulon of E. amylovora was tagged, and its nucleotide sequence was determined. Five open reading frames, with the genes scrK, scrY, scrA, scrB, and scrR, had high homology to genes of the scr regulons from Klebsiella pneumoniae and plasmid pUR400. scrB and scrR of E. amylovora were fused to a histidine tag and to the maltose-binding protein (MalE) of E. coli, respectively. ScrB (53 kDa) catalyzed the hydrolysis of sucrose with a K(m) of 125 mM. Binding of a MalE-ScrR fusion protein to an scrYAB promoter fragment was shown by gel mobility shifts. This complex dissociated in the presence of fructose but not after addition of sucrose. Expression of the scr regulon was studied with an scrYAB promoter-green fluorescent protein gene fusion and measured by flow cytometry and spectrofluorometry. The operon was affected by catabolite repression and induced by sucrose or fructose. The level of gene induction correlated to the sucrose concentration in plant tissue, as shown by flow cytometry. Sucrose mutants created by site-directed mutagenesis did not produce significant fire blight symptoms on apple seedlings, indicating the importance of sucrose metabolism for colonization of host plants by E. amylovora. |
| | |
Authors:
|
J Bogs; K Geider |
Related Documents
:
|
20333176 - Coevolution of dna uptake sequences and bacterial proteomes. 12225586 - Neat: a domain duplicated in genes near the components of a putative fe3+ siderophore t... 17920076 - Cross-talk between iron and nitrogen regulatory networks in anabaena (nostoc) sp. pcc 7... 11471056 - Atp6h, a subunit of vacuolar atpase involved in metal transport: evaluation in canine c... 1588906 - Identification of the structural genes for n-acetylmuramoyl-l-alanine amidase and its m... 6194996 - Precursor nucleotides at the 5' end are not required for processing by rnase e at the 3... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of bacteriology Volume: 182 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 2000 Oct |
Date Detail:
|
Created Date: 2000-10-24 Completed Date: 2000-10-24 Revised Date: 2013-04-18 |
Medline Journal Info:
|
Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 5351-8 Citation Subset: IM |
Affiliation:
|
Max-Planck-Institut für Zellbiologie, Rosenhof, D-68526 Ladenburg, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Bacterial Proteins* Cloning, Molecular Erwinia / genetics*, growth & development, pathogenicity Escherichia coli Proteins* Fructokinases / genetics*, metabolism Gene Expression Regulation, Bacterial Genes, Bacterial* Glycoside Hydrolases / genetics*, metabolism Mutagenesis, Site-Directed Phosphoenolpyruvate Sugar Phosphotransferase System / genetics*, metabolism Porins / genetics*, metabolism Regulon* Repressor Proteins / genetics*, metabolism Sucrose / metabolism* Transcriptional Activation Virulence beta-Fructofuranosidase |
| Chemical | |
Reg. No./Substance:
|
0/Bacterial Proteins; 0/Escherichia coli Proteins; 0/Porins; 0/Repressor Proteins; 0/ScrY protein, E coli; 0/ScrY protein, bacteria; 0/scrR protein, bacteria; 57-50-1/Sucrose; EC 2.7.1.-/Fructokinases; EC 2.7.1.-/Phosphoenolpyruvate Sugar Phosphotransferase System; EC 2.7.1.-/phosphoenolpyruvate-sucrose phosphotransferase; EC 2.7.1.4/fructokinase; EC 3.2.1.-/Glycoside Hydrolases; EC 3.2.1.26/beta-Fructofuranosidase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Regulation of vibrio cholerae genes required for acid tolerance by a member of the "ToxR-like" famil...
Next Document: Binding of ferric enterobactin by the Escherichia coli periplasmic protein FepB.