Document Detail

Molecular analysis of rice plants harboring a multi-functional T-DNA tagging system.
MedLine Citation:
PMID:  19447374     Owner:  NLM     Status:  MEDLINE    
About 25,000 rice T-DNA insertional mutant lines were generated using the vector pCAS04 which has both promoter-trapping and activation-tagging function. Southern blot analysis revealed that about 40% of these mutants were single copy integration and the average T-DNA insertion number was 2.28. By extensive phenotyping in the field, quite a number of agronomically important mutants were obtained. Histochemical GUS assay with 4,310 primary mutants revealed that the GUS-staining frequency was higher than that of the previous reports in various tissues and especially high in flowers. The T-DNA flanking sequences of some mutants were isolated and the T-DNA insertion sites were mapped to the rice genome. The flanking sequence analysis demonstrated the different integration pattern of the right border and left border into rice genome. Compared with Arabidopsis and poplar, it is much varied in the T-DNA border junctions in rice.
Yimian Ma; Luo Liu; Chengguang Zhu; Changhui Sun; Bo Xu; Jun Fang; Jiuyou Tang; Anding Luo; Shouyun Cao; Gupo Li; Qian Qian; Yongbiao Xue; Chengcai Chu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of genetics and genomics = Yi chuan xue bao     Volume:  36     ISSN:  1673-8527     ISO Abbreviation:  J Genet Genomics     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-05-18     Completed Date:  2009-06-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101304616     Medline TA:  J Genet Genomics     Country:  China    
Other Details:
Languages:  eng     Pagination:  267-76     Citation Subset:  IM    
State Key Laboratory of Plant Genomics and National Plant Gene Research Center (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
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MeSH Terms
Chromosomes, Plant / genetics
DNA, Bacterial / genetics*
Genes, Reporter
Genetic Vectors / genetics
Genome, Plant
Glucuronidase / genetics,  metabolism
Mutagenesis, Insertional*
Oryza sativa / genetics*,  growth & development,  metabolism
Rhizobium / genetics,  metabolism
Transformation, Genetic
Reg. No./Substance:
0/DNA, Bacterial; 0/T-DNA; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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