Document Detail


Molecular signature and in vivo behavior of bone marrow endosteal and subendosteal stromal cell populations and their relevance to hematopoiesis.
MedLine Citation:
PMID:  22841688     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the bone marrow cavity, hematopoietic stem cells (HSC) have been shown to reside in the endosteal and subendosteal perivascular niches, which play specific roles on HSC maintenance. Although cells with long-term ability to reconstitute full hematopoietic system can be isolated from both niches, several data support a heterogenous distribution regarding the cycling behavior of HSC. Whether this distinct behavior depends upon the role played by the stromal populations which distinctly create these two niches is a question that remains open. In the present report, we used our previously described in vivo assay to demonstrate that endosteal and subendosteal stromal populations are very distinct regarding skeletal lineage differentiation potential. This was further supported by a microarray-based analysis, which also demonstrated that these two stromal populations play distinct, albeit complementary, roles in HSC niche. Both stromal populations were preferentially isolated from the trabecular region and behave distinctly in vitro, as previously reported. Even though these two niches are organized in a very close range, in vivo assays and molecular analyses allowed us to identify endosteal stroma (F-OST) cells as fully committed osteoblasts and subendosteal stroma (F-RET) cells as uncommitted mesenchymal cells mainly represented by perivascular reticular cells expressing high levels of chemokine ligand, CXCL12. Interestingly, a number of cytokines and growth factors including interleukin-6 (IL-6), IL-7, IL-15, Hepatocyte growth factor (HGF) and stem cell factor (SCF) matrix metalloproteases (MMPs) were also found to be differentially expressed by F-OST and F-RET cells. Further microarray analyses indicated important mechanisms used by the two stromal compartments in order to create and coordinate the "quiescent" and "proliferative" niches in which hematopoietic stem cells and progenitors reside.
Authors:
Alex Balduino; Valeria Mello-Coelho; Zhou Wang; Russell S Taichman; Paul H Krebsbach; Ashani T Weeraratna; Kevin G Becker; Wallace de Mello; Dennis D Taub; Radovan Borojevic
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't     Date:  2012-07-27
Journal Detail:
Title:  Experimental cell research     Volume:  318     ISSN:  1090-2422     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-09-18     Completed Date:  2013-02-11     Revised Date:  2014-03-26    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2427-37     Citation Subset:  IM    
Copyright Information:
Published by Elsevier Inc.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bone Marrow / metabolism,  physiology*
Bone and Bones / cytology,  metabolism,  physiology
Cell Differentiation / genetics,  physiology
Cells, Cultured
Chemokine CXCL12 / genetics,  metabolism
Gene Expression Profiling / methods
Hematopoiesis / genetics,  physiology*
Hematopoietic Stem Cells / cytology,  metabolism,  physiology*
Hepatocyte Growth Factor / genetics,  metabolism
Interleukins / genetics,  metabolism
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Stem Cell Factor / genetics,  metabolism
Stem Cell Niche / genetics,  physiology
Stromal Cells / cytology,  metabolism,  physiology*
Grant Support
ID/Acronym/Agency:
CA-93900/CA/NCI NIH HHS; DK082481/DK/NIDDK NIH HHS; P01 CA093900/CA/NCI NIH HHS; Z99 AG999999/AG/NIA NIH HHS; ZIA AG000766-11/AG/NIA NIH HHS
Chemical
Reg. No./Substance:
0/Chemokine CXCL12; 0/Cxcl12 protein, mouse; 0/Interleukins; 0/Stem Cell Factor; 67256-21-7/Hepatocyte Growth Factor
Comments/Corrections

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