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Molecular Properties of Water-Unextractable Proteoglycans from Hypsizygus marmoreus and Their in Vitro Immunomodulatory Activities.
MedLine Citation:
PMID:  22202808     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Four proteoglycans were sequentially extracted from Hypsizygus marmoreus using 0.1 M NaOH (alkali-soluble proteoglycans [F1] and alkali-insoluble proteoglycans [F3]) and 0.1 M HCl (acid-soluble proteoglycans [F2] and acid-insoluble proteoglycans [F4]), and their structures and immunomodulatory activities were investigated. The proteoglycans were found to contain carbohydrates (19.8-82.4%) with various amounts of proteins (7.7-67.3%), and glucose was the major monosaccharide unit present, along with trace amounts of galactose. The molecular weights (Mw) and the radius of gyration (Rg) of these proteoglycans showed ranges of 16 × 104-19,545 × 104 g/mol and 35-148 nm, respectively, showing significant variations in their molecular conformations. The backbones of F1 and F2 were mainly connected through a-(1→3), (1→4) and b-(1→6)-glycosidic linkages with some branches. The F1 and F2 proteoglycans significantly stimulated Raw264.7 cells to release nitric oxide (NO), prostaglandin E2 (PGE(2)) and various cytokines, such as IL-1β, TNF-α and IL-6 by inducing their mRNA expressions.
Authors:
Hong Hui Bao; Mehdi Tarbasa; Hee Mun Chae; Sang Guan You
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Publication Detail:
Type:  Journal Article     Date:  2011-12-27
Journal Detail:
Title:  Molecules (Basel, Switzerland)     Volume:  17     ISSN:  1420-3049     ISO Abbreviation:  Molecules     Publication Date:  2011  
Date Detail:
Created Date:  2011-12-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100964009     Medline TA:  Molecules     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  207-26     Citation Subset:  IM    
Affiliation:
Department of Marine Food Science and Technology, Gangneung-Wonju National University, 120 Gangneung Daehangno, Gangneung, Gangwon, 210-702, Korea. cheemun6606@hotmail.com.
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