| Molecular Phenotyping of Aging in Single Yeast Cells Using a Novel Microfluidic Device. | |
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MedLine Citation:
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PMID: 22498653 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Budding yeast has served as an important model organism for aging research, and previous genetic studies have led to the discovery of conserved genes/pathways that regulate lifespan across species. However, the molecular causes of aging and death remain elusive, since it is very difficult to directly observe the cellular and molecular events accompanying aging in single yeast cells by the traditional approach based on micro-manipulation. We have developed a microfluidic system to track individual mother cells throughout their lifespan, allowing automated lifespan measurement and direct observation of cell cycle dynamics, cell/organelle morphologies, and various molecular markers. We found that aging of the wild type cells is characterized by an increased general stress and a progressive lengthening of the cell cycle for the last few cell divisions; these features are much less apparent in the long-lived FOB1 deletion mutant. Following the fate of individual cells revealed that there are different forms of cell death that are characterized by different terminal cell morphologies, and associated with different levels of stress and lifespan. We have identified a molecular marker - the level of the expression of Hsp104, as a good predictor for the lifespan of individual cells. Our approach allows detailed molecular phenotyping of single cells in the process of aging and thus provides new insight into its mechanism. © 2012 The Authors Aging Cell © 2012 Blackwell Publishing Ltd/Anatomical Society of Great Britain and Ireland. |
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Authors:
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Zhengwei Xie; Yi Zhang; Ke Zou; Onn Brandman; Chunxiong Luo; Qi Ouyang; Hao Li |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-4-12 |
Journal Detail:
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Title: Aging cell Volume: - ISSN: 1474-9726 ISO Abbreviation: - Publication Date: 2012 Apr |
Date Detail:
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Created Date: 2012-4-13 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101130839 Medline TA: Aging Cell Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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© 2012 The Authors Aging Cell © 2012 Blackwell Publishing Ltd/Anatomical Society of Great Britain and Ireland. |
Affiliation:
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Center for Theoretical Biology, and the State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics, Peking University, Beijing 100871, China Department of Biochemistry and Biophysics and California Institute for Quantitative Biosciences, University of California, San Francisco, CA 94143, USA Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94143, USA. |
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