Document Detail


Molecular phenotyping of aging in single yeast cells using a novel microfluidic device.
MedLine Citation:
PMID:  22498653     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Budding yeast has served as an important model organism for aging research, and previous genetic studies have led to the discovery of conserved genes/pathways that regulate lifespan across species. However, the molecular causes of aging and death remain elusive, because it is very difficult to directly observe the cellular and molecular events accompanying aging in single yeast cells by the traditional approach based on micromanipulation. We have developed a microfluidic system to track individual mother cells throughout their lifespan, allowing automated lifespan measurement and direct observation of cell cycle dynamics, cell/organelle morphologies, and various molecular markers. We found that aging of the wild-type cells is characterized by an increased general stress and a progressive lengthening of the cell cycle for the last few cell divisions; these features are much less apparent in the long-lived FOB1 deletion mutant. Following the fate of individual cells revealed that there are different forms of cell death that are characterized by different terminal cell morphologies, and associated with different levels of stress and lifespan. We have identified a molecular marker - the level of the expression of Hsp104, as a good predictor for the lifespan of individual cells. Our approach allows detailed molecular phenotyping of single cells in the process of aging and thus provides new insight into its mechanism.
Authors:
Zhengwei Xie; Yi Zhang; Ke Zou; Onn Brandman; Chunxiong Luo; Qi Ouyang; Hao Li
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-05-17
Journal Detail:
Title:  Aging cell     Volume:  11     ISSN:  1474-9726     ISO Abbreviation:  Aging Cell     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-07-18     Completed Date:  2012-11-13     Revised Date:  2014-04-01    
Medline Journal Info:
Nlm Unique ID:  101130839     Medline TA:  Aging Cell     Country:  England    
Other Details:
Languages:  eng     Pagination:  599-606     Citation Subset:  IM    
Copyright Information:
© 2012 The Authors. Aging Cell © 2012 Blackwell Publishing Ltd/Anatomical Society of Great Britain and Ireland.
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MeSH Terms
Descriptor/Qualifier:
DNA-Binding Proteins / genetics,  metabolism
Equipment Design
Genes, Fungal
Microfluidic Analytical Techniques*
Models, Biological
Mutation
Phenotype
Saccharomyces cerevisiae / cytology*,  genetics,  metabolism*
Saccharomyces cerevisiae Proteins / genetics,  metabolism
Stress, Physiological
Time Factors
Grant Support
ID/Acronym/Agency:
P50 GM081879/GM/NIGMS NIH HHS; R01 GM070808/GM/NIGMS NIH HHS; R01 GM070808/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/FOB1 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins
Comments/Corrections

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