Document Detail

Molecular and phenotypic characterization of Listeria monocytogenes from U.S. Department of Agriculture Food Safety and Inspection Service surveillance of ready-to-eat foods and processing facilities.
MedLine Citation:
PMID:  20501037     Owner:  NLM     Status:  MEDLINE    
A panel of 501 Listeria monocytogenes isolates obtained from the U.S. Department of Agriculture Food Safety and Inspection Service monitoring programs for ready-to-eat (RTE) foods were subtyped by multilocus genotyping (MLGT) and by sequencing the virulence gene inlA, which codes for internalin. MLGT analyses confirmed that clonal lineages associated with previous epidemic outbreaks were rare (7.6%) contaminants of RTE meat and poultry products and their production environments. Conversely, sequence analyses revealed mutations leading to 11 different premature stop codons (PMSCs) in inlA, including three novel PMSC mutations, and revealed that the frequency of these virulence-attenuating mutations among RTE isolates (48.5%) was substantially higher than previously appreciated. Significant differences (P < 0.001) in the frequency of inlA PMSCs were observed between lineages and between major serogroups, which could partially explain differences in association of these subtypes with human listeriosis. Interrogation of single-nucleotide polymorphisms responsible for PMSCs in inlA improved strain resolution among isolates with the 10 most common pulsed-field gel electrophoresis (PFGE) patterns, 8 of which included isolates with a PMSC in inlA. The presence or absence of PMSCs in inlA accounted for significant differences (P < 0.05) in Caco-2 invasion efficiencies among isolates with identical PFGE patterns, and the proportion of PulseNet entries from clinical sources was significantly higher (P < 0.001) for PFGE patterns exclusively from isolates with full-length inlA. These results indicated that integration of PFGE and DNA sequence-based subtyping provides an improved framework for prediction of relative risk associated with L. monocytogenes strains from RTE foods.
Todd J Ward; Peter Evans; Martin Wiedmann; Thomas Usgaard; Sherry E Roof; Steven G Stroika; Kelley Hise
Related Documents :
22723037 - Impact of food intake, ultrasound transducer, breathing maneuvers and body position on ...
15993967 - Prevalence and location of listeria monocytogenes in farmed rainbow trout.
20156087 - Attribution of human listeria monocytogenes infections in england and wales to ready-to...
16814987 - Detection of listeria monocytogenes in food using a combined enrichment/real-time pcr m...
17225727 - Seal and whale meat: two newly recognized food allergies.
11518607 - Cell transformation in vitro by food-derived heterocyclic amines trp-p-1, trp-p-2 and n...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of food protection     Volume:  73     ISSN:  0362-028X     ISO Abbreviation:  J. Food Prot.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-26     Completed Date:  2010-06-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  861-9     Citation Subset:  IM    
Bacterial Foodborne Pathogens and Mycology Research Unit, U.S. Department of Agriculture, Agricultural Research Service, Peoria, Illinois 61604, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Bacterial Proteins / genetics*
Bacterial Typing Techniques
Caco-2 Cells
Codon, Nonsense
DNA, Bacterial / chemistry,  genetics*
Electrophoresis, Gel, Pulsed-Field
Food Contamination / analysis*
Food Microbiology
Listeria monocytogenes / classification*,  genetics
Meat Products / microbiology
Polymorphism, Single Nucleotide*
Poultry Products / microbiology
Sequence Analysis, DNA
Virulence Factors / genetics*
Reg. No./Substance:
0/Bacterial Proteins; 0/Codon, Nonsense; 0/DNA, Bacterial; 0/Virulence Factors; 139948-71-3/internalin protein, Bacteria

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Virulence properties and characterization of aeromonads isolated from foods of animal origin and env...
Next Document:  Diversity of bacillus species isolated from okpehe, a traditional fermented soup condiment from Nige...