Document Detail

The molecular pathogenicity of Fusarium keratitis: a fungal transcriptional regulator promotes hyphal penetration of the cornea.
MedLine Citation:
PMID:  20856109     Owner:  NLM     Status:  MEDLINE    
PURPOSE: The pathogenic mechanisms of fungal infection during human keratomycosis were investigated in an ex vivo corneal model that used strains of Fusarium oxysporum differing in the production of a fungal transcription factor.
METHODS: A pacC loss-of-function mutant and a pacC dominant-activating mutant were constructed from a wild-type isolate of F. oxysporum, and the 3 strains were characterized by in vitro growth kinetics. Twenty-seven human donor corneas maintained in tissue culture were superficially scarified and topically inoculated with the wild-type, the pacC loss-of-function mutant, or the pacC dominant-activating strains. Relative hyphal invasion into the stroma was compared histopathologically in corneal sections.
RESULTS: F. oxysporum strains demonstrated comparable exponential growth rates in vitro. Wild-type F. oxysporum invaded into the corneal tissue within 1 day and penetrated through the anterior stroma during the next 4 days. The pacC loss-of-function mutant invaded explanted corneas significantly less than the wild-type strain on day 1 (P < 0.0001) and on day 3 (P = 0.0003). The pacC dominant-activating strain adhered and penetrated explanted corneas similar to the wild-type strain.
CONCLUSIONS: The PacC pathway regulating the transcription of fungal genes allows fungal adaptation to the ocular surface and enables invasion of the injured cornea by F. oxysporum.
Xia Hua; Xiaoyong Yuan; Antonio Di Pietro; Kirk R Wilhelmus
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cornea     Volume:  29     ISSN:  1536-4798     ISO Abbreviation:  Cornea     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-11-24     Completed Date:  2011-02-10     Revised Date:  2014-09-08    
Medline Journal Info:
Nlm Unique ID:  8216186     Medline TA:  Cornea     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1440-4     Citation Subset:  IM    
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MeSH Terms
Cornea / microbiology*
Corneal Ulcer / microbiology*
Eye Infections, Fungal / microbiology
Fungal Proteins / genetics*
Fusarium / genetics,  pathogenicity*
Gene Expression Regulation, Fungal / physiology*
Genes, Fungal / physiology
Hyphae / physiology*
Models, Biological
Mycoses / microbiology
Transcription Factors / physiology*
Grant Support
EY02520/EY/NEI NIH HHS; P30 EY002520/EY/NEI NIH HHS; P30 EY002520-30/EY/NEI NIH HHS
Reg. No./Substance:
0/Fungal Proteins; 0/Transcription Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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