Document Detail


Molecular Mechanisms of Therapeutic Hypothermia on Neurological Function In A Swine Model Of Cardiopulmonary Resuscitation.
MedLine Citation:
PMID:  22245747     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
OBJECTIVE: To explore the molecular mechanisms by which mild hypothermia following resuscitation improves neurological function in a porcine model of cardiac arrest. METHODS: Thirty-three inbred Chinese Wuzhishan (WZS) minipigs were used. After 8min of untreated ventricular fibrillation (VT), survived animals (n=29) were randomly divided into two groups including serum group (n=16) and molecular group (n=13). Serum group animals were used to measure porcine-specific TNF-α, IL-6, IL-10, MMP9, AQP4, TIMP1, NSE and S100B at 0.5h, 6h, 12h, 24h and 72h recovery by ELISA. Molecular group animals were used to measure cerebral cortex mRNA and protein expression of NF-κB, MMP9, and AQP4 by real-time quantitative PCR and western blotting at 24h and 72h recovery. Animals were further divided into either normothermia or hypothermia groups. Hypothermia (33°C) was maintained for 12h using an endovascular cooling device. Swine neurologic deficit scores (NDS) were used to evaluate neurological function at 24h and 72h recovery. RESULTS: Twenty-nine of thirty-three (87.9%) animals were successfully resuscitated. The hypothermia group exhibited higher survival rates at 24h (75%) and 72h (62.5%) compared to the normothermia group (37.5% and 25%, respectively). Hypothermia markedly inhibited expression of NF-κB, TNF-α, MMP9, and NSE, and promoted expression of TIMP1 (p<0.01). The mean NDS at 24h and 72h recovery was 112.5 and 61, respectively, in the hypothermic group, and 230 and 207.5, respectively, in the normothermia group. CONCLUSION: Brain protection induced by hypothermia involves inhibition of inflammatory and brain edema pathways.
Authors:
Hong Zhao; Chun-Sheng Li; Ping Gong; Zi-Ren Tang; Rong Hua; Xue Mei; Ming-Yue Zhang; Juan Cui
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-11
Journal Detail:
Title:  Resuscitation     Volume:  -     ISSN:  1873-1570     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0332173     Medline TA:  Resuscitation     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier Ireland Ltd.
Affiliation:
Department of Emergency, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China.
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