| Molecular imaging of the translocator protein (TSPO) in a pre-clinical model of breast cancer. | |
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MedLine Citation:
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PMID: 19949989 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To quantitatively evaluate the utility of a translocator protein (TSPO)-targeted near-infrared (NIR) probe (NIR-conPK11195) for in vivo molecular imaging of TSPO in breast cancer. PROCEDURES: NIR-conPK11195 uptake and TSPO-specificity were validated in TSPO-expressing human breast adenocarcinoma cells (MDA-MB-231). In vivo NIR-conPK11195 biodistribution and accumulation were quantitatively evaluated in athymic nude mice bearing MDA-MB-231 xenografts. RESULTS: Fluorescence micrographs illustrated intracellular labeling of MDA-MB-231 cells by NIR-conPK11195. Quantitative uptake and competition assays demonstrated dose-dependent (p < 0.001) and TSPO-specific (p < 0.001) NIR-conPK11195 uptake. In vivo, NIR-conPK11195 preferentially labeled MDA-MB-231 tumors with an 11-fold (p < 0.001) and 7-fold (p < 0.001) contrast enhancement over normal tissue and unconjugated NIR dye, respectively. CONCLUSIONS: NIR-conPK11195 appears to be a promising TSPO-targeted molecular imaging agent for visualization and quantification of breast cancer cells in vivo. This research represents the first study to demonstrate the feasibility of TSPO imaging as an alternative breast cancer imaging approach. |
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Authors:
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Shelby K Wyatt; H Charles Manning; Mingfeng Bai; Stephanie N Bailey; Pascal Gallant; Guobin Ma; Laura McIntosh; Darryl J Bornhop |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2009-12-01 |
Journal Detail:
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Title: Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging Volume: 12 ISSN: 1860-2002 ISO Abbreviation: Mol Imaging Biol Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-06 Completed Date: 2010-09-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101125610 Medline TA: Mol Imaging Biol Country: United States |
Other Details:
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Languages: eng Pagination: 349-58 Citation Subset: IM |
Affiliation:
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Vanderbilt University Institute of Imaging Science, Vanderbilt University Medical Center, Nashville, TN, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Breast Neoplasms / diagnosis*, pathology Cell Line, Tumor Cell Survival Female Mice Mice, Inbred BALB C Microscopy, Fluorescence Models, Biological* Molecular Imaging / methods* Molecular Probes / chemistry, pharmacokinetics Receptors, GABA / metabolism* Spectroscopy, Near-Infrared Time Factors Tissue Distribution Whole Body Imaging |
| Grant Support | |
ID/Acronym/Agency:
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K25 CA127349/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Molecular Probes; 0/Receptors, GABA; 0/TSPO protein, human |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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