| Molecular imaging of matrix metalloproteinase activation to predict murine aneurysm expansion in vivo. | |
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MedLine Citation:
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PMID: 20554725 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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METHODS: We used a model of carotid aneurysm in apolipoprotein E-deficient (apoE(-/-)) mice. Radiotracers with specificity for activated MMPs were used to detect and quantify MMP activation by micro-SPECT/CT in vivo. Tracer uptake was confirmed by autoradiography and gamma-well counting, and specificity was demonstrated using an excess of unlabeled precursor and a specific MMP inhibitor. RESULTS: We demonstrated that several MMPs are expressed with distinct temporal patterns in aneurysm. Significant focal uptake was observed in aneurysmal carotid arteries, peaking at 4 wk after aneurysm induction. In a group of animals imaged serially at 2 and 4 wk after aneurysm induction, MMP tracer uptake at 2 wk correlated well with the vessel area assessed by histology at 4 wk. CONCLUSION: Molecular imaging of MMP activation is a useful experimental, and potentially clinical, tool to noninvasively predict the propensity of an aneurysm to expansion in vivo. |
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Authors:
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Mahmoud Razavian; Jiasheng Zhang; Lei Nie; Sina Tavakoli; Niema Razavian; Lawrence W Dobrucki; Albert J Sinusas; D Scott Edwards; Michael Azure; Mehran M Sadeghi |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-06-16 |
Journal Detail:
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Title: Journal of nuclear medicine : official publication, Society of Nuclear Medicine Volume: 51 ISSN: 1535-5667 ISO Abbreviation: J. Nucl. Med. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-07-02 Completed Date: 2010-07-27 Revised Date: 2011-08-01 |
Medline Journal Info:
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Nlm Unique ID: 0217410 Medline TA: J Nucl Med Country: United States |
Other Details:
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Languages: eng Pagination: 1107-15 Citation Subset: IM |
Affiliation:
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Cardiovascular Molecular Imaging Laboratory, Yale University School of Medicine, New Haven, Connecticut, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apolipoproteins E / genetics Arteriovenous Fistula / enzymology*, radionuclide imaging* Autoradiography Carotid Arteries / radionuclide imaging Catalysis Disease Progression Enzyme Activation Immunohistochemistry Indicators and Reagents Matrix Metalloproteinases / biosynthesis, metabolism* Mice Mice, Knockout Radiopharmaceuticals / diagnostic use Reverse Transcriptase Polymerase Chain Reaction Substrate Specificity Tomography, Emission-Computed Tomography, Emission-Computed, Single-Photon |
| Grant Support | |
ID/Acronym/Agency:
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HL70295/HL/NHLBI NIH HHS; P01 HL070295-09/HL/NHLBI NIH HHS; R01 HL085093-03/HL/NHLBI NIH HHS; R01 HL085093-05/HL/NHLBI NIH HHS; R01 HL85093/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Apolipoproteins E; 0/Indicators and Reagents; 0/Radiopharmaceuticals; EC 3.4.24.-/Matrix Metalloproteinases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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