Document Detail


Molecular imaging of matrix metalloproteinase activation to predict murine aneurysm expansion in vivo.
MedLine Citation:
PMID:  20554725     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
METHODS: We used a model of carotid aneurysm in apolipoprotein E-deficient (apoE(-/-)) mice. Radiotracers with specificity for activated MMPs were used to detect and quantify MMP activation by micro-SPECT/CT in vivo. Tracer uptake was confirmed by autoradiography and gamma-well counting, and specificity was demonstrated using an excess of unlabeled precursor and a specific MMP inhibitor.
RESULTS: We demonstrated that several MMPs are expressed with distinct temporal patterns in aneurysm. Significant focal uptake was observed in aneurysmal carotid arteries, peaking at 4 wk after aneurysm induction. In a group of animals imaged serially at 2 and 4 wk after aneurysm induction, MMP tracer uptake at 2 wk correlated well with the vessel area assessed by histology at 4 wk.
CONCLUSION: Molecular imaging of MMP activation is a useful experimental, and potentially clinical, tool to noninvasively predict the propensity of an aneurysm to expansion in vivo.
Authors:
Mahmoud Razavian; Jiasheng Zhang; Lei Nie; Sina Tavakoli; Niema Razavian; Lawrence W Dobrucki; Albert J Sinusas; D Scott Edwards; Michael Azure; Mehran M Sadeghi
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2010-06-16
Journal Detail:
Title:  Journal of nuclear medicine : official publication, Society of Nuclear Medicine     Volume:  51     ISSN:  1535-5667     ISO Abbreviation:  J. Nucl. Med.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-07-02     Completed Date:  2010-07-27     Revised Date:  2014-09-13    
Medline Journal Info:
Nlm Unique ID:  0217410     Medline TA:  J Nucl Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1107-15     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Apolipoproteins E / genetics
Arteriovenous Fistula / enzymology*,  radionuclide imaging*
Autoradiography
Carotid Arteries / radionuclide imaging
Catalysis
Disease Progression
Enzyme Activation
Immunohistochemistry
Indicators and Reagents
Matrix Metalloproteinases / biosynthesis,  metabolism*
Mice
Mice, Knockout
Radiopharmaceuticals / diagnostic use
Reverse Transcriptase Polymerase Chain Reaction
Substrate Specificity
Tomography, Emission-Computed
Tomography, Emission-Computed, Single-Photon
Grant Support
ID/Acronym/Agency:
HL70295/HL/NHLBI NIH HHS; P01 HL070295/HL/NHLBI NIH HHS; P01 HL070295-09/HL/NHLBI NIH HHS; R01 HL085093/HL/NHLBI NIH HHS; R01 HL085093-03/HL/NHLBI NIH HHS; R01 HL085093-05/HL/NHLBI NIH HHS; R01 HL85093/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Apolipoproteins E; 0/Indicators and Reagents; 0/Radiopharmaceuticals; EC 3.4.24.-/Matrix Metalloproteinases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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