Document Detail


The Molecular Events Involved in Oligodendrocyte Precursor Cell Proliferation Induced by the Conditioned Medium from B104 Neuroblastoma Cells.
MedLine Citation:
PMID:  23283697     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The conditioned medium from B104 neuroblastoma cells (B104CM) induces proliferation of oligodendrocyte progenitor cells (OPCs) in vitro. However, the molecular events that occur during B104CM-induced proliferation of OPCs has not been well clarified. In the present study, using OPCs immunopanned from embryonic day 14 Sprague-Dawley rat spinal cords, we explored the activation of several signaling pathways and the expression of several important immediate early genes (IEGs) and cyclins in OPCs in response to B104CM. We found that B104CM can induce OPC proliferation through the activation of the extracellular signal-regulated kinases 1 and 2 (Erk1/2), but not PI3K or p38 MAPK signaling pathways in vitro. The IEGs involved in B104CM-induced OPC proliferation include c-fos, c-jun and Id2, but not c-myc, fyn, or p21. The cyclins D1, D2 and E are also involved in B104CM-stimulated proliferation of OPCs. The activation of Erk results in subsequent expression of IEGs (such as c-fos, c-jun and Id-2) and cyclins (including cyclin D1, D2 and E), which play key roles in cell cycle initiation and OPC proliferation. Collectively, these results suggest that the phosphorylation of Erk1/2 is an important molecular event during OPC proliferation induced by B104CM.
Authors:
Jian-Guo Hu; Xing-Jun Wu; Yi-Fan Feng; Gang-Ming Xi; Ling-Xiao Deng; Zhen-Huan Wang; Rui Wang; Lin Shen; Jian-Sheng Zhou; He-Zuo Lü
Related Documents :
23452857 - Encoding and transducing the synaptic or extrasynaptic origin of nmda receptor signals ...
9485467 - Change of ganglioside accessibility at the plasma membrane surface of cultured neurons,...
24421757 - Neuroprotective effects of resveratrol and epigallocatechin gallate polyphenols are med...
6097467 - The phosphorylation of bovine and human lens polypeptides.
15305037 - Role of ca2+/calmodulin signaling pathway on morphological development of candida albic...
667417 - Effects of droperidol on activity of carotid body chemoreceptors in cat.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-3
Journal Detail:
Title:  Neurochemical research     Volume:  -     ISSN:  1573-6903     ISO Abbreviation:  Neurochem. Res.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7613461     Medline TA:  Neurochem Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Clinical Laboratory Science, The First Affiliated Hospital of Bengbu Medical College, 287 Chang Huai Road, Bengbu, 233004, People's Republic of China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Hippocampal Asymmetry: Differences in Structures and Functions.
Next Document:  Effects of Ethanol Exposure During Early Pregnancy in Hyperactive, Inattentive and Impulsive Behavio...