Document Detail

Molecular dynamics studies on T1 lipase: insight into a double-flap mechanism.
MedLine Citation:
PMID:  20443585     Owner:  NLM     Status:  MEDLINE    
T1 lipase is isolated from the palm Geobacillus zalihae strain T1 in Malaysia, functioning as a secreted protein responsible for the catalyzing hydrolysis of long-chain triglycerides into fatty acids and glycerol at high temperatures. In the current study, using 30 ns molecular dynamics simulations at different temperatures, an aqueous activation was detected for T1 lipase. This aqueous activation in T1 lipase was mainly caused by a double-flap movement mechanism. The double flaps were constituted by the hydrophobic helices 6 and 9. Helix 6 employed two major components with the hydrophilic part at the surface and the hydrophobic part inside. In the aqueous solution, the hydrophobic part could provide enough power for helix 6 to move away, driving the protein into an open configuration and exposing the catalytic triad. Our findings could provide structural evidence to support the double-flap movement, revealing the catalytic mechanism for T1 lipase.
Ying Wang; Dong-Qing Wei; Jing-Fang Wang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chemical information and modeling     Volume:  50     ISSN:  1549-960X     ISO Abbreviation:  J Chem Inf Model     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-24     Completed Date:  2010-08-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101230060     Medline TA:  J Chem Inf Model     Country:  United States    
Other Details:
Languages:  eng     Pagination:  875-8     Citation Subset:  IM    
College of Life Science and Biotechnology, Shanghai Jiaotong University, 800 Dongchuan Road, Shanghai 200240, China.
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MeSH Terms
Geobacillus / enzymology*
Lipase / chemistry*
Molecular Dynamics Simulation*
Protein Conformation
Reg. No./Substance:

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