| Molecular cytogenetic characterization of the human cerebral microvessel endothelial cell line hCMEC/D3. | |
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MedLine Citation:
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PMID: 19864871 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The immortalized human cerebral microvessel endothelial cell line hCMEC/D3 has been repeatedly used as a model of human blood-brain barrier (BBB). hCMEC/D3 cells between passage 25 and 35 are most often applied in research, remained phenotypically nontransformed, and cells maintained many characteristics of human brain endothelial cells. Also hCMEC/D3 was thought to have conserved a normal diploid karyotype over all these passages. Here we characterized the cell line using high-resolution multicolor fluorescence in situ hybridization (FISH) approaches and revealed a complex karyotype in the 30th passage. Clonal cryptic unbalanced structural rearrangements and numerical aberrations were discovered and described as follows: 45 approximately 48,XX, -X,del(5)(q11)[2],del(9)(q11)[3],+9[3],del(11)(q13 approximately 14)[2], der(14)t(14;21)(q32.33;q22.3)[28],der(15)t(9;15)(p11;p11)[13], dup(15)(p11q11)[5],der(21)t(17;21)(p12;q22)[9],-22[6][cp28]. In summary, a complex karyotype with clonal unbalanced chromosomal rearrangements is present in hCMEC/D3. Thus, we solicit to include molecular cytogenetics in the testing of all cell lines prior to application of their use in complex studies. |
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Authors:
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H Mkrtchyan; S Scheler; I Klein; A Fahr; P O Couraud; I A Romero; B Weksler; T Liehr |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-10-26 |
Journal Detail:
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Title: Cytogenetic and genome research Volume: 126 ISSN: 1424-859X ISO Abbreviation: Cytogenet. Genome Res. Publication Date: 2009 |
Date Detail:
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Created Date: 2010-01-29 Completed Date: 2010-02-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101142708 Medline TA: Cytogenet Genome Res Country: Switzerland |
Other Details:
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Languages: eng Pagination: 313-7 Citation Subset: IM |
Copyright Information:
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Copyright 2009 S. Karger AG, Basel. |
Affiliation:
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Jena University Hospital, Institute of Human Genetics and Anthropology, Jena, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Brain
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blood supply*,
cytology* Cell Line Chromosome Aberrations Endothelial Cells / cytology* Humans Karyotyping Microvessels / cytology* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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