Document Detail


Molecular cytogenetic characterization of the human cerebral microvessel endothelial cell line hCMEC/D3.
MedLine Citation:
PMID:  19864871     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The immortalized human cerebral microvessel endothelial cell line hCMEC/D3 has been repeatedly used as a model of human blood-brain barrier (BBB). hCMEC/D3 cells between passage 25 and 35 are most often applied in research, remained phenotypically nontransformed, and cells maintained many characteristics of human brain endothelial cells. Also hCMEC/D3 was thought to have conserved a normal diploid karyotype over all these passages. Here we characterized the cell line using high-resolution multicolor fluorescence in situ hybridization (FISH) approaches and revealed a complex karyotype in the 30th passage. Clonal cryptic unbalanced structural rearrangements and numerical aberrations were discovered and described as follows: 45 approximately 48,XX, -X,del(5)(q11)[2],del(9)(q11)[3],+9[3],del(11)(q13 approximately 14)[2], der(14)t(14;21)(q32.33;q22.3)[28],der(15)t(9;15)(p11;p11)[13], dup(15)(p11q11)[5],der(21)t(17;21)(p12;q22)[9],-22[6][cp28]. In summary, a complex karyotype with clonal unbalanced chromosomal rearrangements is present in hCMEC/D3. Thus, we solicit to include molecular cytogenetics in the testing of all cell lines prior to application of their use in complex studies.
Authors:
H Mkrtchyan; S Scheler; I Klein; A Fahr; P O Couraud; I A Romero; B Weksler; T Liehr
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-10-26
Journal Detail:
Title:  Cytogenetic and genome research     Volume:  126     ISSN:  1424-859X     ISO Abbreviation:  Cytogenet. Genome Res.     Publication Date:  2009  
Date Detail:
Created Date:  2010-01-29     Completed Date:  2010-02-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101142708     Medline TA:  Cytogenet Genome Res     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  313-7     Citation Subset:  IM    
Copyright Information:
Copyright 2009 S. Karger AG, Basel.
Affiliation:
Jena University Hospital, Institute of Human Genetics and Anthropology, Jena, Germany.
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MeSH Terms
Descriptor/Qualifier:
Brain / blood supply*,  cytology*
Cell Line
Chromosome Aberrations
Endothelial Cells / cytology*
Humans
Karyotyping
Microvessels / cytology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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