| Molecular cloning and functional characterization of the avian macrophage migration inhibitory factor (MIF). | |
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MedLine Citation:
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PMID: 20470818 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Macrophage migration inhibitory factor (MIF) is recognized as a soluble factor produced by sensitized T lymphocytes and inhibits the random migration of macrophages. Recent studies have revealed a more prominent role for MIF as a multi-functional cytokine mediating both innate and adaptive immune responses. This study describes the cloning and functional characterization of avian MIF in an effort to better understand its role in innate and adaptive immunity, and potential use in poultry health applications. The full-length avian MIF gene was amplified from stimulated chicken lymphocytes and cloned into a prokaryotic expression vector. The confirmed 115 amino acid sequence of avian MIF has 71% identity with human and murine MIF. The bacterially expressed avian recombinant MIF (rChMIF) was purified, followed by endotoxin removal, and then tested by chemotactic assay and quantitative real-time PCR (qRT-PCR). Diff-Quick staining revealed a substantial decrease in migration of macrophages in the presence of 0.01microg/ml rChMIF. qRT-PCR analysis revealed that the presence of rChMIF enhanced levels of IL-1beta and iNOS during PBMCs stimulation with LPS. Additionally, the Con A-stimulated lymphocytes showed enhanced interferon (IFN)-gamma and IL-2 transcripts in the presence of rChMIF. Interestingly, addition of rChMIF to the stimulated PBMCs, in the presence of lymphocytes, showed anti-inflammatory function of rChMIF. To our knowledge, this study represents the first report for the functional characterization of avian MIF, demonstrating the inhibition of macrophage migration, similar to mammalian MIF, and the mediation of inflammatory responses during antigenic stimulation. |
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Authors:
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Sungwon Kim; Kate B Miska; Mark C Jenkins; Ray H Fetterer; Chasity M Cox; Lindsay H Stuard; Rami A Dalloul |
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Publication Detail:
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Type: Journal Article Date: 2010-05-20 |
Journal Detail:
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Title: Developmental and comparative immunology Volume: 34 ISSN: 1879-0089 ISO Abbreviation: Dev. Comp. Immunol. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-07-08 Completed Date: 2011-02-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7708205 Medline TA: Dev Comp Immunol Country: United States |
Other Details:
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Languages: eng Pagination: 1021-32 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Avian Immunobiology Laboratory, Department of Animal and Poultry Sciences, Virginia Tech, 3170 Litton-Reaves, Blacksburg, VA 24061, United States. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adaptive Immunity
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genetics Amino Acid Sequence Animals Birds / genetics*, immunology* Cell Migration Assays, Macrophage Cell Movement / drug effects, immunology Cells, Cultured Cloning, Molecular Coculture Techniques Humans Immunity, Innate / genetics Macrophage Migration-Inhibitory Factors / genetics, immunology, metabolism*, pharmacology Macrophages / immunology, metabolism*, pathology Mice Molecular Sequence Data Sequence Homology, Amino Acid T-Lymphocytes / drug effects, immunology, metabolism*, pathology |
| Chemical | |
Reg. No./Substance:
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0/Macrophage Migration-Inhibitory Factors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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