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Molecular Cloning and Expression Analysis of the Starch-branching Enzyme III Gene from Common Wheat (Triticum aestivum ).
MedLine Citation:
PMID:  23344688     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The starch-branching enzyme (SBE) catalyzes the formation of branch points by cleaving the α-1,4 linkage in polyglucans and reattaching the chain via an α-1,6 linkage. Three types of SBE isoforms (SBEI, SBEII, and SBEIII) exist in higher plants, with the number of SBE isoforms being species-specific. This study isolated the SBEIII cDNA sequence (3,780 bp), designated TaSBEIII (accession no. JQ346193), from common wheat (Triticum aestivum L.) using the RACE method, revealing that the SBEIII gene exists in common wheat. The open reading frame of TaSBEIII was 2,748 bp. The predicted protein of 916 amino acids contained the specific characteristics of the SBEIII protein: four highly conserved regions and a central (α/β)(8) barrel domain. The SBE activity of the protein expressed in Escherichia coli (BL21) was also measured and verified. During the wheat grain filling period, TaSBEIII was constitutively expressed. The role of the TaSBEIII gene in starch synthesis is discussed.
Authors:
Guozhang Kang; Shuyi Li; Mengqin Zhang; Huifang Peng; Chenyang Wang; Yunji Zhu; Tiancai Guo
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-24
Journal Detail:
Title:  Biochemical genetics     Volume:  -     ISSN:  1573-4927     ISO Abbreviation:  Biochem. Genet.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0126611     Medline TA:  Biochem Genet     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Key Laboratory of Physiology, Ecology and Genetic Improvement of Food Crops in Henan Province, National Engineering Research Centre for Wheat, Henan Agricultural University, Zhengzhou, 450002, China, zkangg@163.com.
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