Document Detail


Molecular characterization of the glauce mutant: a central cell-specific function is required for double fertilization in Arabidopsis.
MedLine Citation:
PMID:  22872756     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Double fertilization of the egg cell and the central cell by two sperm cells, resulting in the formation of the embryo and the endosperm, respectively, is a defining characteristic of flowering plants. The Arabidopsis thaliana female gametophytic mutant glauce (glc) can exhibit embryo development without any endosperm. Here, we show that in glc mutant embryo sacs one sperm cell successfully fuses with the egg cell but the second sperm cell fails to fuse with the central cell, resulting in single fertilization. Complementation studies using genes from the glc deletion interval identified an unusual genomic locus having homology to BAHD (for BEAT, AHCT, HCBT, and DAT) acyl-transferases with dual transcription units and alternative splicing that could rescue the sterility defect of glc. Expression of these transcripts appears restricted to the central cell, and expression within the central cell is sufficient to restore fertility. We conclude that the central cell actively promotes its own fertilization by the sperm cell through a signaling mechanism involving products of At1g65450. Successful fertilization of the egg cell is not blocked in the glc mutant, suggesting that evolution of double fertilization in flowering plants involved acquisition of specific functions by the central cell to enable its role as a second female gamete.
Authors:
Yehoram Leshem; Cameron Johnson; Samuel E Wuest; Xiaoya Song; Quy A Ngo; Ueli Grossniklaus; Venkatesan Sundaresan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-08-07
Journal Detail:
Title:  The Plant cell     Volume:  24     ISSN:  1532-298X     ISO Abbreviation:  Plant Cell     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-09-28     Completed Date:  2013-02-13     Revised Date:  2013-08-12    
Medline Journal Info:
Nlm Unique ID:  9208688     Medline TA:  Plant Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3264-77     Citation Subset:  IM    
Affiliation:
Department of Plant Biology, University of California, Davis, California 95616, USA.
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MeSH Terms
Descriptor/Qualifier:
Acyltransferases / genetics,  metabolism
Alternative Splicing
Amino Acid Sequence
Arabidopsis / cytology,  embryology*,  genetics
Arabidopsis Proteins / genetics,  metabolism
Endosperm / cytology,  genetics,  metabolism
Evolution, Molecular
Fertilization*
Gene Expression Regulation, Plant*
Genes, Plant
Genetic Complementation Test
Genetic Loci
Germ Cells, Plant / cytology,  metabolism
Green Fluorescent Proteins / metabolism
Molecular Sequence Data
Organ Specificity
Phylogeny
Seeds / cytology*,  genetics,  metabolism
Transcriptome
Chemical
Reg. No./Substance:
0/Arabidopsis Proteins; 147336-22-9/Green Fluorescent Proteins; EC 2.3.-/Acyltransferases
Comments/Corrections

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