Document Detail

A Molecular Beacon-Based Signal-Off Surface-Enhanced Raman Scattering Strategy for Highly Sensitive, Reproducible, and Multiplexed DNA Detection.
MedLine Citation:
PMID:  23359560     Owner:  NLM     Status:  Publisher    
Stem-loop oligonucleotides tagged with organic dyes are employed as capture and reporter probes in a molecular beacon-based signal-off surface- enhanced Raman scattering (SERS) strategy. DNA strands are immobilized on a gold nanoparticle-decorated silicon nanowire array (AuNPs@SiNWAr). DNA with a concentration down to ≈10 fM is detected under irradiation from a low-power laser, which is better than or comparable to that detected by signal-on SERS methods. The signal-off strategy allows identification of single-base mismatches and detection of multiplexed DNA.
Xinpan Wei; Shao Su; Yuanyuan Guo; Xiangxu Jiang; Yiling Zhong; Yuanyuan Su; Chunhai Fan; Shuit-Tong Lee; Yao He
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-29
Journal Detail:
Title:  Small (Weinheim an der Bergstrasse, Germany)     Volume:  -     ISSN:  1613-6829     ISO Abbreviation:  Small     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101235338     Medline TA:  Small     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Institute of Functional Nano & Soft Materials (FUNSOM) and Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Soochow University, Suzhou 215123, PR China.
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