| Molecular assay for screening and quantifying DNA in biological evidence: the modified Q-TAT assay. | |
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MedLine Citation:
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PMID: 20384933 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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A method is described for the quantitation of total human and male DNA. Q-TAT utilizes end-point, multiplex polymerase chain reaction (PCR) amplification of the amelogenin and SRY loci to quantify DNA and incorporates a cloned nonhuman template to detect PCR inhibition. Standard curves of fluorescence from amelogenin or SRY amplicons were generated from amplification of known amounts of NIST traceable SRM-female or SRM-male DNA. Curves showed good linearity up to 500 pg of SRM-template (R(2) > 0.99) and reliably estimated total and male DNA content in casework samples. The nonhuman pRL(null) template included in each PCR was a sensitive indicator of known PCR inhibitors including EDTA, hemin, blue denim dye, and humic acid. Finally, the SRY amplicon was a sensitive indicator of male DNA and, in mixtures, could reliably estimate male DNA present in an excess of female DNA. The Q-TAT multiplex is a reliable quantitation method for forensic DNA typing. |
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Authors:
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Jon Wilson; Valerie Fuller; Gifty Benson; Denise Juroske; Eric Duvall; Jun Fu; Jane Pritchard; Robert W Allen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-08 |
Journal Detail:
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Title: Journal of forensic sciences Volume: 55 ISSN: 1556-4029 ISO Abbreviation: J. Forensic Sci. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-07-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0375370 Medline TA: J Forensic Sci Country: United States |
Other Details:
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Languages: eng Pagination: 1050-7 Citation Subset: IM |
Affiliation:
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Police Laboratory, Tulsa Police Department, Tulsa, OK, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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