Document Detail


A molecular analysis of fecal and mucosal bacterial communities in irritable bowel syndrome.
MedLine Citation:
PMID:  19693670     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: The objectives of this study were, firstly, to determine the diversity of the host's gut microbiota in irritable bowel syndrome (IBS) using a culture-independent method (DGGE of the 16S rRNA gene) and, secondly, to examine mucosal biopsies of IBS patients and compare them to their own fecal microbiota. METHODS: The diversity of the dominant microbiota in the fecal material of IBS patients was compared to a healthy control group. In addition, we compared the mucosal and fecal microbiota of IBS patients. RESULTS: Statistical analysis of the mean similarity data for these groups indicated a significant difference (P < 0.001) between IBS (n = 47) and healthy controls (n = 33) with significantly more variation in the gut microbiota of healthy volunteers than that of IBS patients. The average intra-individual similarity between the mucosa and luminal microbiota was 84%, which indicates that different communities were present at the two sites. This difference, however, is similar to that previously described between these two niches in control subjects. The average inter-individual similarity of the bacterial communities on the mucosa and in the lumen of IBS was not significantly different (P > 0.05). CONCLUSIONS: IBS impacts equally on both bacterial communities in the IBS host and a significant difference in the gut microbiota exists between fecal samples from IBS patients and healthy controls. The reason for this difference is unclear and various possible explanations are available, but much more work is required to determine the underlying reason for this observation.
Authors:
Caroline Codling; Liam O'Mahony; Fergus Shanahan; Eamonn M M Quigley; Julian R Marchesi
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-08-20
Journal Detail:
Title:  Digestive diseases and sciences     Volume:  55     ISSN:  1573-2568     ISO Abbreviation:  Dig. Dis. Sci.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-01-12     Completed Date:  2010-02-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7902782     Medline TA:  Dig Dis Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  392-7     Citation Subset:  AIM; IM    
Affiliation:
Department of Medicine, Alimentary Pharmabiotic Centre, Cork University Hospital, Cork, Ireland.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Bacteria / genetics*,  isolation & purification
Biopsy
Colon / microbiology,  pathology
DNA, Bacterial / analysis*
Feces / microbiology*
Female
Humans
Intestinal Mucosa / microbiology*,  pathology
Irritable Bowel Syndrome / microbiology*,  pathology
Metagenome
Middle Aged
Polymerase Chain Reaction
Young Adult
Chemical
Reg. No./Substance:
0/DNA, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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