Document Detail


Modulatory effects of testosterone on 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced neurotoxicity.
MedLine Citation:
PMID:  8263548     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this experiment, we examined the modulatory effects of testosterone on the parkinsonism-inducing drug 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in two strains of mice. Orchidectomized male CD-1 and C57/B1 mice were implanted with either empty Silastic capsules or capsules containing testosterone and subsequently treated with MPTP. A small area of the corpus striatum was removed for determination of dopamine (DA) content, whereas the remainder was superfused and used to measure L-DOPA (5 microM)-evoked DA release. In animals treated with MPTP, L-DOPA-evoked DA release was reduced significantly in CD-1 mice, but not in C57/B1 mice, treated with testosterone. No differences in L-DOPA-stimulated DA release between MPTP-versus vehicle-treated mice was observed in either the CD-1 or C57/B1 mice receiving empty Silastic capsules. Corpus striatum DA contents were more severely depleted in the MPTP-sensitive C57/B1 versus the CD-1 mouse strain irrespective of hormone treatment. These results confirm previous results demonstrating differences in these two mouse strains in response to the neurotoxic effects of MPTP upon corpus striatum DA content. More interestingly, they show an important differential modulatory effect of testosterone upon L-DOPA-evoked DA release as a function of MPTP treatment and indicate that testosterone significantly alters the neurotoxic effects of MPTP in the CD-1 mouse.
Authors:
D Dluzen; R Jain; B Liu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neurochemistry     Volume:  62     ISSN:  0022-3042     ISO Abbreviation:  J. Neurochem.     Publication Date:  1994 Jan 
Date Detail:
Created Date:  1994-01-24     Completed Date:  1994-01-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  94-101     Citation Subset:  IM    
Affiliation:
Department of Anatomy, Northeastern Ohio Universities, College of Medicine, Rootstown 44272-0095.
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MeSH Terms
Descriptor/Qualifier:
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine / antagonists & inhibitors
Animals
Corpus Striatum / drug effects,  metabolism*
Dopamine / metabolism*
Drug Implants
Levodopa / pharmacology
MPTP Poisoning*
Male
Mice
Mice, Inbred C57BL
Mice, Inbred Strains
Neurotoxins / antagonists & inhibitors,  toxicity*
Orchiectomy
Parkinson Disease, Secondary / chemically induced,  physiopathology*
Reference Values
Species Specificity
Testosterone / administration & dosage,  pharmacology*
Chemical
Reg. No./Substance:
0/Drug Implants; 0/Levodopa; 0/Neurotoxins; 28289-54-5/1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; 58-22-0/Testosterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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