Document Detail

Modulatory effects of connexin-43 expression on gap junction intercellular communications with mast cells and fibroblasts.
MedLine Citation:
PMID:  21328609     Owner:  NLM     Status:  MEDLINE    
The influence of mast cells upon aberrant wound repair and excessive fibrosis has supportive evidence, but the mechanism for these mast cell activities is unclear. It is proposed that heterocellular gap junction intercellular communication (GJIC) between fibroblasts and mast cells directs some fibroblast activities. An in vitro model was used employing a rodent derived peritoneal mast cell line (RMC-1) and human dermal derived fibroblasts. The influence of the expression of the gap junction channel structural protein, connexin 43 (Cx-43) on heterocellular GJIC, the expression of microtubule β-tubulin and microfilament α smooth muscle actin (SMA) were investigated. The knockdown of Cx-43 by siRNA in RMC-1 cells completely blocked GJIC between RMC-1 cells. SiRNA knockdown of Cx-43 within fibroblasts only dampened GJIC between fibroblasts. It appears Cx-43 is the only expressed connexin (Cx) in RMC-1 cells. Fibroblasts express other Cxs that participate in GJIC between fibroblasts in the absence of Cx-43 expression. Heterocellular GJIC between RMC-1 cells and fibroblasts transformed fibroblasts into myofibroblasts, expressing α SMA within cytoplasmic stress fibers. The knockdown of Cx-43 in RMC-1 cells increased β-tubulin expression, but its knockdown in fibroblasts reduced β-tubulin expression. Knocking down the expression of Cx-43 in fibroblasts limited αSMA expression. Cx-43 participation is critical for heterocellular GJIC between mast cells and fibroblasts, which may herald a novel direction for controlling fibrosis.
Ashley L Pistorio; H Paul Ehrlich
Related Documents :
8314909 - Matrix metalloproteinase-9 (92 kda gelatinase/type iv collagenase) from u937 monoblasto...
7560079 - Phorbol esters induce death in mcf-7 breast cancer cells with altered expression of pro...
7700359 - Defective signalling through the t- and b-cell antigen receptors in lymphoid cells lack...
6581139 - Phenotypic changes induced in the human thymic all cell line hpb-all by an ingenol este...
3032049 - Hormone-like activity of human thrombin.
2027529 - Diacylglycerol formation and dna synthesis in endothelin-stimulated rat c6 glioma cells...
7916009 - Estramustine: a novel radiation enhancer in human carcinoma cells.
11792359 - Biocytin and biotin uptake into nb2a neuroblastoma and c6 astrocytoma cells.
18483299 - Inhibition of the akt/survivin pathway synergizes the antileukemia effect of nutlin-3 i...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Journal of cellular biochemistry     Volume:  112     ISSN:  1097-4644     ISO Abbreviation:  J. Cell. Biochem.     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-04-05     Completed Date:  2011-07-20     Revised Date:  2013-09-20    
Medline Journal Info:
Nlm Unique ID:  8205768     Medline TA:  J Cell Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1441-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Wiley-Liss, Inc.
Division of Plastic Surgery, Department of Surgery, Milton S. Hershey Medical Center, Hershey, Pennsylvania 17033, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Actins / genetics
Cell Culture Techniques
Connexin 43 / genetics,  physiology*
Fibroblasts / cytology,  metabolism,  physiology*
Gap Junctions / genetics
Gene Expression
Keloid / genetics,  metabolism
Mast Cells / cytology,  metabolism,  physiology*
RNA, Small Interfering / genetics
Tubulin / genetics,  physiology
Wounds and Injuries / genetics,  pathology
Grant Support
Reg. No./Substance:
0/Actins; 0/Connexin 43; 0/RNA, Small Interfering; 0/Tubulin; 0/smooth muscle actin, rat

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Comparison of procedures to assess non-linear and time-varying effects in multivariable models for s...
Next Document:  PIK3 and ERK/Nrf2 pathways are involved in oleanolic acid-induced heme oxygenase-1 expression in rat...