Document Detail


Modulatory effects of connexin-43 expression on gap junction intercellular communications with mast cells and fibroblasts.
MedLine Citation:
PMID:  21328609     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The influence of mast cells upon aberrant wound repair and excessive fibrosis has supportive evidence, but the mechanism for these mast cell activities is unclear. It is proposed that heterocellular gap junction intercellular communication (GJIC) between fibroblasts and mast cells directs some fibroblast activities. An in vitro model was used employing a rodent derived peritoneal mast cell line (RMC-1) and human dermal derived fibroblasts. The influence of the expression of the gap junction channel structural protein, connexin 43 (Cx-43) on heterocellular GJIC, the expression of microtubule β-tubulin and microfilament α smooth muscle actin (SMA) were investigated. The knockdown of Cx-43 by siRNA in RMC-1 cells completely blocked GJIC between RMC-1 cells. SiRNA knockdown of Cx-43 within fibroblasts only dampened GJIC between fibroblasts. It appears Cx-43 is the only expressed connexin (Cx) in RMC-1 cells. Fibroblasts express other Cxs that participate in GJIC between fibroblasts in the absence of Cx-43 expression. Heterocellular GJIC between RMC-1 cells and fibroblasts transformed fibroblasts into myofibroblasts, expressing α SMA within cytoplasmic stress fibers. The knockdown of Cx-43 in RMC-1 cells increased β-tubulin expression, but its knockdown in fibroblasts reduced β-tubulin expression. Knocking down the expression of Cx-43 in fibroblasts limited αSMA expression. Cx-43 participation is critical for heterocellular GJIC between mast cells and fibroblasts, which may herald a novel direction for controlling fibrosis.
Authors:
Ashley L Pistorio; H Paul Ehrlich
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Journal of cellular biochemistry     Volume:  112     ISSN:  1097-4644     ISO Abbreviation:  J. Cell. Biochem.     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-04-05     Completed Date:  2011-07-20     Revised Date:  2013-09-20    
Medline Journal Info:
Nlm Unique ID:  8205768     Medline TA:  J Cell Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1441-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Wiley-Liss, Inc.
Affiliation:
Division of Plastic Surgery, Department of Surgery, Milton S. Hershey Medical Center, Hershey, Pennsylvania 17033, USA.
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MeSH Terms
Descriptor/Qualifier:
Actins / genetics
Animals
Cell Culture Techniques
Connexin 43 / genetics,  physiology*
Fibroblasts / cytology,  metabolism,  physiology*
Fibrosis
Gap Junctions / genetics
Gene Expression
Humans
Keloid / genetics,  metabolism
Mast Cells / cytology,  metabolism,  physiology*
RNA, Small Interfering / genetics
Rats
Tubulin / genetics,  physiology
Wounds and Injuries / genetics,  pathology
Grant Support
ID/Acronym/Agency:
GM-056851/GM/NIGMS NIH HHS; R01 GM056851/GM/NIGMS NIH HHS; R01 GM056851-10/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Actins; 0/Connexin 43; 0/RNA, Small Interfering; 0/Tubulin; 0/smooth muscle actin, rat
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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