| Modulatory effects of black tea polyphenols on oxidant-antioxidant profile and expression of proliferation, apoptosis, and angiogenesis-associated proteins in the rat forestomach carcinogenesis model. | |
| | |
MedLine Citation:
|
PMID: 17530359 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: Chemoprevention by dietary constituents has emerged as a novel approach to control stomach cancer incidence. We therefore evaluated the chemopreventive effects of black tea polyphenols (Polyphenon-B) on oxidant-antioxidant status, cell proliferation, apoptosis, and angiogenesis during N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced gastric carcinogenesis. METHODS: Male Wistar rats were divided into four groups. Rats in group 1 and 2 were given MNNG (150 mg/kg body weight) by intragastric intubation three times at 2 week intervals and followed for 26 weeks. Rats in group 2 received in addition a basal diet containing 0.05% Polyphenon-B. Group 3 animals were given 0.05% Polyphenon-B alone. Group 4 animals served as controls. The status of lipid peroxidation and antioxidants and the expression of the lipid peroxidation marker 4-hydroxy nonenal (4-HNE), proliferating cell nuclear antigen (PCNA), glutathiones-transferase (GST)-pi, Bcl-2, Bax, cytochrome C, caspase 3, cytokeratins, and vascular endothelial growth factor (VEGF) were used as biomarkers. RESULTS: Intragastric administration of MNNG induced well-differentiated squamous cell carcinomas that showed diminished lipid and protein oxidation and an increase in antioxidant status. This was associated with increased cell proliferation, angiogenesis, and invasive potential coupled with apoptosis evasion as revealed by upregulation of PCNA, GST-pi, Bcl-2, cytokeratins, and VEGF and downregulation of Bax, cytochrome C, and caspase 3 protein expression. Dietary administration of Polyphenon-B effectively suppressed MNNG-induced gastric carcinogenesis, as evidenced by modulation of oxidant-antioxidant status, inhibition of cell proliferation and infiltration, and angiogenesis associated with apoptosis induction. CONCLUSIONS: The present study provides evidence that Polyphenon-B exerts multifunctional inhibitory effects on MNNG-induced gastric carcinogenesis and suggests that it can be developed as a potential chemopreventive agent. |
| | |
Authors:
|
Ramalingam Senthil Murugan; Kurapathy Venkata Poorna Chandra Mohan; Koji Uchida; Yukihiko Hara; Duvuru Prathiba; Siddavaram Nagini |
Related Documents
:
|
23139429 - Inhibition of meningitis-associated neutrophil apoptosis by tnf-α depends on functiona... 23557269 - Importance of reverse signaling of the tnf superfamily in immune regulation. 10927169 - Reactive oxygen species, cell signaling, and cell injury. 23319119 - The interplay between fiber and the intestinal microbiome in the inflammatory response. 23162759 - Tlr3/ticam-1 signaling in tumor cell rip3-dependent necroptosis. 23562609 - Microrna-17/20a/106a modulate macrophage inflammatory responses through targeting signa... 7954799 - Dna damage can induce apoptosis in proliferating lymphoid cells via p53-independent mec... 20878249 - Iron and immunity: immunological consequences of iron deficiency and overload. 11514079 - Methionine-enkephalin-and dynorphin a-release from immune cells and control of inflamma... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-05-25 |
Journal Detail:
|
Title: Journal of gastroenterology Volume: 42 ISSN: 0944-1174 ISO Abbreviation: J. Gastroenterol. Publication Date: 2007 May |
Date Detail:
|
Created Date: 2007-05-28 Completed Date: 2007-07-19 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9430794 Medline TA: J Gastroenterol Country: Japan |
Other Details:
|
Languages: eng Pagination: 352-61 Citation Subset: IM |
Affiliation:
|
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar 608 002, Tamil Nadu, India. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Aldehydes
/
metabolism Animals Antioxidants / metabolism* Apoptosis Cell Proliferation Chemoprevention Disease Models, Animal Down-Regulation / physiology Immunohistochemistry Lipid Peroxidation Male Methylnitronitrosoguanidine Oxidants / metabolism* Oxidation-Reduction Phenols / pharmacology* Proliferating Cell Nuclear Antigen / metabolism Rats Rats, Wistar Stomach / pathology Thiobarbituric Acid Reactive Substances Up-Regulation / physiology |
| Chemical | |
Reg. No./Substance:
|
0/Aldehydes; 0/Antioxidants; 0/Oxidants; 0/Phenols; 0/Proliferating Cell Nuclear Antigen; 0/Thiobarbituric Acid Reactive Substances; 0/polyphenon B; 29343-52-0/4-hydroxy-2-nonenal; 70-25-7/Methylnitronitrosoguanidine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Association of tannase-producing Staphylococcus lugdunensis with colon cancer and characterization o...
Next Document: A randomized trial of induction doses of interferon alone or in combination with ribavirin or ribavi...