Document Detail

Modulatory effects of black tea polyphenols on oxidant-antioxidant profile and expression of proliferation, apoptosis, and angiogenesis-associated proteins in the rat forestomach carcinogenesis model.
MedLine Citation:
PMID:  17530359     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Chemoprevention by dietary constituents has emerged as a novel approach to control stomach cancer incidence. We therefore evaluated the chemopreventive effects of black tea polyphenols (Polyphenon-B) on oxidant-antioxidant status, cell proliferation, apoptosis, and angiogenesis during N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced gastric carcinogenesis. METHODS: Male Wistar rats were divided into four groups. Rats in group 1 and 2 were given MNNG (150 mg/kg body weight) by intragastric intubation three times at 2 week intervals and followed for 26 weeks. Rats in group 2 received in addition a basal diet containing 0.05% Polyphenon-B. Group 3 animals were given 0.05% Polyphenon-B alone. Group 4 animals served as controls. The status of lipid peroxidation and antioxidants and the expression of the lipid peroxidation marker 4-hydroxy nonenal (4-HNE), proliferating cell nuclear antigen (PCNA), glutathiones-transferase (GST)-pi, Bcl-2, Bax, cytochrome C, caspase 3, cytokeratins, and vascular endothelial growth factor (VEGF) were used as biomarkers. RESULTS: Intragastric administration of MNNG induced well-differentiated squamous cell carcinomas that showed diminished lipid and protein oxidation and an increase in antioxidant status. This was associated with increased cell proliferation, angiogenesis, and invasive potential coupled with apoptosis evasion as revealed by upregulation of PCNA, GST-pi, Bcl-2, cytokeratins, and VEGF and downregulation of Bax, cytochrome C, and caspase 3 protein expression. Dietary administration of Polyphenon-B effectively suppressed MNNG-induced gastric carcinogenesis, as evidenced by modulation of oxidant-antioxidant status, inhibition of cell proliferation and infiltration, and angiogenesis associated with apoptosis induction. CONCLUSIONS: The present study provides evidence that Polyphenon-B exerts multifunctional inhibitory effects on MNNG-induced gastric carcinogenesis and suggests that it can be developed as a potential chemopreventive agent.
Ramalingam Senthil Murugan; Kurapathy Venkata Poorna Chandra Mohan; Koji Uchida; Yukihiko Hara; Duvuru Prathiba; Siddavaram Nagini
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-05-25
Journal Detail:
Title:  Journal of gastroenterology     Volume:  42     ISSN:  0944-1174     ISO Abbreviation:  J. Gastroenterol.     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-05-28     Completed Date:  2007-07-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9430794     Medline TA:  J Gastroenterol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  352-61     Citation Subset:  IM    
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar 608 002, Tamil Nadu, India.
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MeSH Terms
Aldehydes / metabolism
Antioxidants / metabolism*
Cell Proliferation
Disease Models, Animal
Down-Regulation / physiology
Lipid Peroxidation
Oxidants / metabolism*
Phenols / pharmacology*
Proliferating Cell Nuclear Antigen / metabolism
Rats, Wistar
Stomach / pathology
Thiobarbituric Acid Reactive Substances
Up-Regulation / physiology
Reg. No./Substance:
0/Aldehydes; 0/Antioxidants; 0/Oxidants; 0/Phenols; 0/Proliferating Cell Nuclear Antigen; 0/Thiobarbituric Acid Reactive Substances; 0/polyphenon B; 29343-52-0/4-hydroxy-2-nonenal; 70-25-7/Methylnitronitrosoguanidine

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