Document Detail

Modulatory effects of achatin-I, an Achatina endogenous neuroactive peptide, on responses to 5-hydroxytryptamine.
MedLine Citation:
PMID:  8095899     Owner:  NLM     Status:  MEDLINE    
Achatin-I (Gly-D-Phe-L-Ala-L-Asp) was found in the ganglia of an African giant snail (Achatina fulica Férussac), and proposed as an excitatory neurotransmitter of Achatina neurones. At 3 x 10(-6) the peptide markedly enhanced the fast inward current (Iin) of an Achatina neurone type, TAN (tonically autoactive neurone), produced by the pneumatic pressure ejection of 5-HT. This Iin was facilitated immediately by the achatin-I perfusion, and the facilitation decreased gradually even with the peptide present. The dose (duration)-response curves of the TAN fast Iin on pressure ejection in the absence (control) and presence of achatin-I at 3 x 10(-6) M (n = 8) were analyzed as follows. The ED50 (95% confidence limit) were 59.3 ms (13.9-95.3 ms) for the control, and 36.5 ms (19.5-52.6 ms) for achatin-I. The Emax were 1.06 +/- 0.11 nA for the control, and 1.74 +/- 0.26 nA for achatin-I (P < 0.01 for paired data). Among achatin-I derivatives, achatin-II (Gly-L-Phe-L-Ala-L-Asp) enhanced the TAN fast response to 5-HT, but was ten times weaker than achatin-I. [L-Glu4]achatin-I (Gly-D-Phe-L-Ala-L-Glu) and achatin-I amide (Gly-D-Phe-Ala-L-Asp-NH2) had no facilitatory effect. We propose that achatin-I is a neuromodulator as well as a neurotransmitter for Achatina giant neurones.
G J Liu; H Takeuchi
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of pharmacology     Volume:  231     ISSN:  0014-2999     ISO Abbreviation:  Eur. J. Pharmacol.     Publication Date:  1993 Feb 
Date Detail:
Created Date:  1993-04-20     Completed Date:  1993-04-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1254354     Medline TA:  Eur J Pharmacol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  259-65     Citation Subset:  IM    
Department of Physiology, Gifu University School of Medicine, Japan.
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MeSH Terms
Amino Acid Sequence
Molecular Sequence Data
Neurons / drug effects
Neuropeptides / pharmacology*
Neurotransmitter Agents / pharmacology*
Serotonin / pharmacology*
Snails / metabolism*
Structure-Activity Relationship
Reg. No./Substance:
0/Neuropeptides; 0/Neurotransmitter Agents; 121912-19-4/achatin I; 50-67-9/Serotonin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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