Document Detail

Modulatory effect of arachidonate 5-lipoxygenation on glomerular cell proliferation in nephrotoxic serum nephritis.
MedLine Citation:
PMID:  8245690     Owner:  NLM     Status:  MEDLINE    
The modulatory effect of arachidonate 5-lipoxygenation on glomerular cell growth was assessed in rat nephrotoxic serum nephritis (NSN). After a single intravenous injection of anti-glomerular basement membrane immune serum, significant increments in glomerular proliferative activity (GPA)--assessed by tritiated thymidine incorporation in short-term glomerular cultures--occurred and were associated with enhanced glomerular cell proliferation, assessed in cortical sections by staining for the presence of proliferating cell nuclear antigen (PCNA) positive cells in glomeruli. Leukocyte depletion induced by x irradiation ameliorated the enhanced GPA and reduced PCNA (+) cell counts. The same effect was observed after treatment of rats with the arachidonate 5-lipoxygenase inhibitor MK886. These observations indicate that in NSN, leukocytes infiltrating glomeruli, and leukocyte-derived arachidonate 5-lipoxygenation eicosanoids promote glomerular cell proliferation.
S H Wu; E A Lianos
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of laboratory and clinical medicine     Volume:  122     ISSN:  0022-2143     ISO Abbreviation:  J. Lab. Clin. Med.     Publication Date:  1993 Dec 
Date Detail:
Created Date:  1994-01-04     Completed Date:  1994-01-04     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375375     Medline TA:  J Lab Clin Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  703-10     Citation Subset:  AIM; IM    
Department of Medicine, Medical College of Wisconsin, Milwaukee.
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MeSH Terms
Arachidonate 5-Lipoxygenase / antagonists & inhibitors,  metabolism*
Basement Membrane / immunology
Cell Count
Cell Division
Glomerular Mesangium / pathology
Glomerulonephritis / immunology,  pathology*
Immune Sera
Indoles / pharmacology
Kidney Glomerulus / immunology,  pathology*
Leukocyte Count
Leukotriene B4 / biosynthesis
Microscopy, Electron
Rats, Sprague-Dawley
Grant Support
Reg. No./Substance:
0/Immune Sera; 0/Indoles; 118414-82-7/L 663536; 71160-24-2/Leukotriene B4; EC 5-Lipoxygenase

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