Document Detail


Modulation of transferrin-receptor activity and recycling after induced differentiation of BeWo choriocarcinoma cells.
MedLine Citation:
PMID:  2400396     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BeWo human choriocarcinoma cells normally grow as cytotrophoblast cells. However, in the presence of 100 microM-forskolin or 5 mM-theophylline, these cells form syncytia similar to morphologically well differentiated syncytiotrophoblasts. We have examined the effect of syncytia formation on transferrin-receptor activity and recycling. Although cellular proliferation stops upon growth in the presence of forskolin or theophylline, the number of cell-surface transferrin-receptors unexpectedly increased 2-fold, whereas the total cellular number increased at most 15%. The rate of biosynthesis of the transferrin receptor as well as class I MHC glycoprotein did not change measurably during syncytium formation. The biosynthesis of human chorionic gonadotropin increased 35-fold after 30 h of growth in the presence of theophylline. The redistribution of the transferrin receptor in syncytia is maintained by a decreased rate constant of endocytosis (0.141 min-1 compared with 0.231 min-1 for control cells) and an increased rate constant of externalization (0.122 min-1 compared with 0.060 min-1 for control cells). These altered rates of endocytosis and externalization resulted in an increased rate of iron accumulation in the syncytia. Furthermore, the recycling time of the transferrin receptor decreased in cells grown in the presence of theophylline (14.6 min compared with 21.2 min in control cells).
Authors:
A van der Ende; A du Maine; A L Schwartz; G J Strous
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Biochemical journal     Volume:  270     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1990 Sep 
Date Detail:
Created Date:  1990-10-12     Completed Date:  1990-10-12     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  451-7     Citation Subset:  IM    
Affiliation:
Department of Cell Biology, University of Utrecht, Medical School, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Cell Differentiation / drug effects
Choriocarcinoma / metabolism,  pathology*
Chorionic Gonadotropin / biosynthesis
Endocytosis
Forskolin / pharmacology
Histocompatibility Antigens Class I / biosynthesis
Humans
Iron / metabolism
Kinetics
Receptors, Transferrin / metabolism*
Theophylline / pharmacology
Transferrin / metabolism
Tumor Cells, Cultured
Grant Support
ID/Acronym/Agency:
GM 38284/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Chorionic Gonadotropin; 0/Histocompatibility Antigens Class I; 0/Receptors, Transferrin; 11096-37-0/Transferrin; 58-55-9/Theophylline; 66428-89-5/Forskolin; 7439-89-6/Iron
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