Document Detail


Modulation of swarming and virulence by fatty acids through the RsbA protein in Proteus mirabilis.
MedLine Citation:
PMID:  15557604     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
After sensing external signals, Proteus mirabilis undergoes a multicellular behavior called swarming which is coordinately regulated with the expression of virulence factors. Here we report that exogenously added fatty acids could act as signals to regulate swarming in P. mirabilis. Specifically, while oleic acid enhanced swarming, some saturated fatty acids, such as lauric acid, myristic acid, palmitic acid, and stearic acid, inhibited swarming. We also found that expression of hemolysin, which has been shown to be coordinately regulated with swarming, was also inhibited by the above saturated fatty acids. Previously we identified a gene, rsbA, which may encode a histidine-containing phosphotransmitter of the bacterial two-component signaling system and act as a repressor of swarming and virulence factor expression in P. mirabilis. We found that while myristic acid, lauric acid, and palmitic acid exerted their inhibitory effect on swarming and hemolysin expression through an RsbA-dependent pathway, the inhibition by stearic acid was mediated through an RsbA-independent pathway. Biofilm formation and extracellular polysaccharide (EPS) production play an important role in P. mirabilis infection. We found that RsbA may act as a positive regulator of biofilm formation and EPS production. Myristic acid was found to slightly stimulate biofilm formation and EPS production, and this stimulation was mediated through an RsbA-dependent pathway. Together, these data suggest that fatty acids may act as environmental cues to regulate swarming and virulence in P. mirabilis and that RsbA may play an important role in this process.
Authors:
Shwu-Jen Liaw; Hsin-Chih Lai; Won-Bo Wang
Related Documents :
412974 - Gastric secretion with feeding and restraint in conscious rhesus monkeys.
7800854 - Neuropeptide y functions as a physiologic regulator of cephalic phase acid secretion.
385834 - Pancreotone, an inhibitor of pancreatic secretion in extracts of ileal and colonic mucosa.
6860304 - Arteriovenous differences for amino acids across control and acid-secreting rat stomach...
7333674 - Factors affecting phaeomelanin production by a melanin-producing (mel) mutant of vibrio...
6414334 - A device for the liberation and determination of 14co2.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Infection and immunity     Volume:  72     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2004-11-23     Completed Date:  2004-12-30     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6836-45     Citation Subset:  IM    
Affiliation:
Graduate Institute of Microbiology, College of Medicine, National Taiwan University, No. 1, Jen-Ai Road, 1st Section, Taipei, Taiwan, Republic of China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / physiology*
Biofilms
Fatty Acids / pharmacology*
Gene Expression Regulation, Bacterial
Polysaccharides, Bacterial / biosynthesis
Proteus mirabilis / drug effects*,  pathogenicity,  physiology
Virulence Factors / analysis
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Fatty Acids; 0/Polysaccharides, Bacterial; 0/RsbA protein, Proteus mirabilis; 0/Virulence Factors
Comments/Corrections
Erratum In:
Infect Immun. 2005 Apr;73(4):2568

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Vesicular transport is not required for the cytoplasmic pool of cholera toxin to interact with the s...
Next Document:  Resistance of Mycoplasma pulmonis to complement lysis is dependent on the number of Vsa tandem repea...